Epidermal Blister Roof

The epidermis was separated from dermis by creating a suction blister. The flattened blister roof was then processed without sectioning and stained for localization of nerve endings (Cy3, recolored green) and Langerhans cells (Cy2, recolored red) using Cy2- and Cy3- conjugated secondary antibodies from Jackson ImmunoResearch Laboratories, Inc. to provide a “bird’s eye view” of the distribution of nerve endings and dendritic cells within the epidermis. Some basal cells are weakly positive for the nerve marker.