Anti-Biotin, Anti-Fluorescein and Anti-Digoxin antibodies

Monoclonal Mouse Anti-Biotin, Anti-Fluorescein and Anti-Digoxin are available in a wide range of conjugate options for direct detection of their target molecules, which are commonly used as tags on proteins and nucleic acids. If signal amplification is desired, these antibodies can be used unconjugated, followed by conjugated anti-mouse IgG (H+L) (see examples below using Mouse Anti-Biotin).

Signal Enhancement with Anti-Biotin antibodies

A. Direct detection of biotinylated primary antibodies with conjugated Mouse Anti-Biotin.
B. Signal enhancement with conjugated Anti-Mouse IgG (H+L).

Detection with Anti-HRP antibodies

Affinity-purified anti-horseradish peroxidase (HRP) may be used to detect HRP or to enhance signal by binding to HRP-conjugated molecules. Anti-HRP also may be used to convert an HRP conjugate into a different signal as illustrated in the example below using ImmunoGold-complexed anti-HRP. Goat Anti-HRP is also available conjugated to alkaline phosphatase and fluorescent probes, providing additional flexibility to the detection method.

In this example, ImmunoGold-complexed Goat Anti-HRP binds to an HRP-conjugated secondary antibody. ImmunoGold Anti-HRP with silver enhancement has been used in place of the HRP substrate diaminobenzidine (DAB) to create less diffused images on tissue sections labeled with HRP reagents (Gee et al., J. Histochem. Cytochem. 1991, 39, 863, Roth et al., Methods in Lab. Invest. 1992. 67, 263).

Other Signal Enhancement Methods