CUTANA™ CUT&Tag Kit

Ultra-sensitive. Rapid results. Rigorously tested.

Cleavage Under Targets and Tagmentation (CUT&Tag) assays use a fusion of protein A, protein G, and Tn5 (pAG-Tn5) to selectively target antibody-labelled chromatin for high-resolution sequencing analysis. These innovations enable low-cost mapping from small numbers of cells – poising CUT&Tag to enable epigenomic studies of unprecedented breadth and scale.

EpiCypher is a leading provider of user-friendly CUT&Tag reagents and workflows. The CUTANA™ CUT&Tag Kit is the result of our rigorous optimization efforts and has been intentionally designed for rapid throughput and accessible pricing. Advantages include:

• Streamlined two-day protocol with no library prep
• Exclusive single-tube workflow maximizes yield
• Reliable profiling using low cell numbers
• Dramatically reduced assay costs
• Comprehensive kit goes from cells to sequence-ready DNA

Robust signal in active and repressed chromatin

High-quality chromatin mapping from low cell numbers

The CUTANA™ CUT&Tag protocol generates data with high signal over background using as few as 10,000 cells and only 5-8 million sequencing reads.

These advances are driven by EpiCypher’s one-tube CUT&Tag workflow, which minimizes sample loss and increases throughput. Combined with low sequencing depth requirements, our kit provides substantial cost savings vs. ChIP-seq.

Reliable workflow supported by robust controls

EpiCypher scientists have developed a user-friendly protocol that is highly consistent across assay replicates. The CUTANA™ CUT&Tag Kit comes with all the controls and guidance you need for successful experiments:

• Control antibodies
• Nucleosome spike-in controls
• Detailed quality control metrics
• Troubleshooting tips

Compatible with diverse sample processing conditions

The CUTANA™ CUT&Tag Kit was used to map H3K4me3 in native (unfixed) nuclei, native cells, and lightly cross-linked nuclei, using protocol adaptations outlined in the manual. Results are consistent across sample preparations, expanding the application of CUT&Tag to additional sample types and targets.

Fast-track your chromatin profiling:

Save time and costs with CUTANA™ CUT&Tag Kits

CUTANA™ CUT&Tag Kit – A Look Inside the Box!

Everything you need for high-quality chromatin mapping

The CUTANA™ CUT&Tag Kit Manual

The CUTANA™ CUT&Tag Manual is a complete guide to CUT&Tag experiments. It leverages EpiCypher’s own research & development to help users plan and execute successful CUT&Tag assays. In addition to our user-friendly protocol, we provide key guidance on:

• Optimizing assays for new cell types, targets, or low cell numbers
• How to identify suitable antibodies for CUT&Tag
• Quality control metrics for before and after sequencing
• Selecting an appropriate sequencing platform
• Troubleshooting difficult assays
• and much more!

The kit also comes with a two-sided protocol quick card to keep at your bench while performing CUT&Tag assays.