Silver Nanoparticles

Cytodiagnostics spherical silver nanoparticles are available with core sizes of 10nm – 100nm.

Our unique silver nanoparticles synthesis and purification protocols consistently produce high quality monodisperse silver nanoparticles with a narrow size distribution (CV <15%) and high purity. Cytodiagnostics nano silver products are ideal for a wide range of applications including photovoltaics, biological sensor development and nanotoxicology studies.

Cytodiagnostics unique Reactant Free Silver Nanoparticles have the same properties as our Standard Silver Nanoparticles with the added benefit of higher purity (99%).

The reactant free formulation is ideal when performing for example toxicity studies by isolating the effects of the nanoparticles while minimizing those from contaminating reactants remaining from the manufacturing process.

Custom Conjugation Services – Nanoparticles

Cytodiagnostics provides an array of options for customers to do their own conjugations. Our gold and silver nanoparticles, and gold nanourchins are optimally designed for efficient passive adsorption of a multitude of proteins, antibodies, thiolated oligos, and more. For covalent conjugates, Cytodiagnostics also provides ready-to-use kits: Gold Conjugation Kits, Silver Conjugation Kits, NanoUrchin Conjugation Kits.

Let Us Do It For You!

Cytodiagnostics custom conjugation services help design and produce your very own custom conjugate. This includes most ligands ranging from proteins to enzymes to oligonucleotides, and this service extends to all the nanoparticles in our expansive catalog. You as a customer tell us the parameters and end-goal you need met, and our team of experts will work out the rest for you.

Silver nanoparticle protein conjugates are a great alternative to traditional gold protein conjugates if peak absorbance is desired in the 400-500nm range.

Cytodiagnostics silver nanoparticle protein conjugates can be used for a variety of applications such as secondary detection agents in immunoblotting, lateral flow/vertical flow devices, ELISA, light microscopy and electron microscopy.

If you are interested in having your own antibody, or other protein conjugated to our silver nanoparticles as a custom service please contact us.

Browse Our Available Silver Nanoparticle Conjugates

Cytodiagnostics NHS-activated Silver Nanoparticle Conjugation Kits have been optimized for high efficiency one-step conjugation of proteins and other primary amine-containing ligands to silver nanoparticles.

The kits contains ready-to-use pre-made mixtures. No activation or manipulation of the silver nanoparticles is required prior to conjugation, which often results in poor performing conjugates. Simply mix your protein with the pre-activated NHS ester silver nanoparticles supplied in the kit to generate your conjugate.

The precisely engineered silver surface coat on our NHS ester-activated silver nanoparticles results in high conjugation efficiency and stable conjugates while minimizing non-specific binding in your assay.

Features & Benefits of NHS-Activated Silver Nanoparticles

• Results in covalently bound ligand and more stable conjugate.
• Fast and convenient one-step conjugation reaction with no pre-activation requirements.
• Spacer between the silver nanoparticle surface and conjugated ligand minimizes effects on the tertiary protein structure, which can lead to poor performing conjugates, a common problem seen in conjugates prepared by passive adsorption.
• Precisely engineering surface coating that reduces non-specific protein binding in biological assays.

Carboxyl functionalized silver nanoparticles are ideal for conjugation of proteins and other primary amine containing ligands using standard EDC/NHS coupling chemistry.

Our carboxylated silver nanoparticles are available in 8 different sizes ranging from 10-100nm, are more than 95% spherical and have uniform size distribution (CV <18%). In addition, the choice of two different length PEG surface spacer allows control of the distance between a conjugated molecule and the silver surface.

Features

• Superior size distribution compared to the leading competitor; available from 10nm to 100nm.
• Precisely engineered surface with an optimized carboxyl group density for easy conjugation.

Applications

• Ideal for development of silver conjugates for use in for example:
• Immunoblotting
• Lateral Flow Assays
• LSPR Assays
• Microscopy Applications (e.g. TEM, Darkfield)

Cytodiagnostics methoxy silver nanoparticles have precisely engineered surface chemistry that renders them inert in most applications. This inert property allows these nanoparticles to be used as excellent control samples when in combination with one of our many other functionalized nanoparticles. In applications where the nanoparticles are not being used as controls, the surface chemistry enables them to be used in solutions with much higher salt concentrations relative to the corresponding standard nanoparticles.
Cytodiagnostics methoxy silver nanoparticles are available with diameters ranging from 10nm – 100nm and are also available with 2 different spacer lengths (2 kDa and 5 kDa), allowing for better optimization.

Cytodiagnostics biotin functionalized silver nanoparticles are available with diameters in the range of 10nm – 100nm and have surface plasmon resonance absorption and scattering characteristics in the 400nm-500nm range. These particles have a precisely engineered surface for optimal streptavidin binding while minimizing non-specific binding to other proteins present in your sample.

The silver nanoparticle label allows for convenient binding and detection of streptavidin in applications such as TEM, ELISA and Immuno dot-blots among others.