The Mini-Beadbeater-1 can also be used for dry grinding. Here, steel beads are added to hard samples such as hair, bone, teeth, seeds and minerals and are completely powdered in 10-60 seconds. Softer materials such as biological tissue, rubber or plastics can be powdered by first pre-freezing the sample to liq.N2 temperatures (Cryo-grinding).
Features
- Rapid and highly efficient.
- Totally Sealed – no dangerous aerosols.
- Simple to use.
- Disposable vials and beads – no sample cross-contamination.
- Very high energy shaking – far superior to lab vortexers. Uses proven, near horizontal shaking in a compressed figure-8 pattern.
- Versatile – use for up to 400 mg (wet weight) of bacteria, yeast, algae, tissue culture cells and plant and animal tissue.
Specifications
- Motor: 115 Volt A/C, 60 Hz, 0.6 amp, 3 wire grounded plug. (European 220 Volt version available)
- Dimensions: 5 inches W, 10 inches D, 7 inches H, shipping wt. 10 lbs
- Digital: Touch pad controls speed and length of homogenization.
- Selectible Oscillations/minute: 2500, 4200, 4600, and 4800.
- Throw or vial displacement 3/4 inches (in horizontal axis).
- Time Setting: 0-5 minutes in 10 second increments.
- Standard 2.0 ml screw-cap microvials with a sharp conical bottom. Not designed for skirted vials.
- Cell or tissue capacity up to 400 mg (wet weight)
Selected Applications Using the Mini-Beadbeater
Extraction from Soil & Wastes
- The Extraction and Purification of Microbial DNA from Sediments, Andrew Ogram et. al., J. Microbiol. Methods, Vol.7, p.57-66 (1987)
- Methods for microbial DNA extraction from soil for PCR amplification, C. Yeates, et. al., Biological Procedures Online (1998)
- Small-scale DNA Sample Preparation Method for Field PCR Detection of Microbial Cells and Spores in Soil, C.R. Kuske, et. al., Appl. Environ. Microbiology, Vol.64, p.2463-2472 (1998)
- Evaluation and Optimistion of DNA Extraction and Purification Procedures for Soil and Sediment Samples, D.N. Miller et. al., Appl. Environ. Microbiology, Vol.65, p.4715-4724 (1999). Click for web copy
- Rapid Method for Coextraction of DNA and RNA from Natural Environments for Analysis of Ribosomal DNA- and rRNA-based Microbial Community Composition, R.I. Griffiths et. al., Appl. Environ. Microbiology, Vol 66, p.5488-5491(2000)
- Simultaneous DNA/RNA Extraction from Soil, a protocol based on a modified method of Griffiths et. al.(2000), from Eoin Brodie, April 2003. References a Fast-Prep machine. Call for MBB equivalent speed settings.
- Improved Extraction of PCR-quality Community DNA from Digesta and Fecal Samples, Zhongtang Yu and Mark Morrison, BioTechniques, Vol 36, p.808-813 (2004). Content: Bead beating gives “1.5 to 6 fold increase in DNA yield compared to other methods”
- Comparitive analysis of fecal DNA extraction methods with phylogenetic microarray: Effective recovery of bacterial and archaeal DNA using mechnical cell lysis, Anne Solonen et al, J Microbiological Methods, Vol 81, p.127-134 (2010). Content: Four widely used methods are compared. DNA yields varied up to 35 fold. A modified protocol of Yu and Morrison was the best.
- A Methods Protocol for the Extraction of DNA from Sediment/Soil Samples
Virus & Spores
- West Nile virus Epidemic in Horses, Tuscany Region, Italy, Gian L Autorino et. al., Emerg. Infect. Dis., Vol.8, No..12, (2002) (serial online),
- Reexamination of the Role of Autolysis in the Development of Myxococcus xanthus, Kathleen A. O’Connor and David R. Zusman, J. Bacteriology, Vol.170, p.4103-4112 (1988)
- A Field Investigation of Bacillus anthracis Contamination of USDA and Other Washington, DC Buildings during the Antrax Attack of October 2001, James A. Higgins, et. al., Appl. Environ. Microbiology, Vol.69, p.593-599 (2003)
Invertebrates
- Disruption of the Nematode Worm C. elegans, A procedural note, Christopher Hopkins, (June 2005).
Hair
- Simultaneous Quantification of Opiates, Cocaine, and Metabolites in Hair by LC-APCI-MS/MS, Karl B. Scheidweiler and Marilyn A. Huestis, Anal. Chem., Vol.76 (15), p.4358–4363 (2004)
Pathogens in Plants
- Comparison of Tissue-Disruption Methods for PCR-Based Detection of Plant Pathogens, Paul Vincelli and Bernadette Amsden, Plant Disease, Vol.97, No.3, p.363-368 (2013)
