iFluor™ Kits

AAT Bioquest’s iFluor™ dyes are Optimised for labelling proteins, in particular, antibodies.   These dyes are bright, photostable and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555 and 633 nm).

List of all available kits

ReadiLink™ iFluor™ 350 Antibody Labelling Kit *Microscale Optimised for Labelling 50 µg Antibody Per Reaction*

iFluor™ 350 dyes have fluorescence excitation and emission maxima of ~350 nm and ~440 nm respectively. These spectral characteristics make them an excellent replacement for AMCA and Alexa Fluor® 350 labelling dye (Alexa Fluor® is the trademark of Invitrogen). ReadiLink™ labelling kits essentially only require 2 simple mixing steps without a column purification needed. iFluor™ 350 SE used in this ReadiLink™ kit is reasonably stable and shows good reactivity and selectivity with protein amino groups. The kit has all the essential components for labelling ~2×50 ug antibody. Each of the two vials of iFluor™ 350 dye provided in the kit is Optimised for labelling ~50 µg antibody. iFluor™ 350 SE protein labelling kit provides a convenient method to label monoclonal, polyclonal antibodies or other proteins (>10 kDa) with the iFluor™ 350 SE.

Detailed Information:   Protocol   References   MSDS   Spectrum

ReadiLink™ iFluor™ 488 Antibody Labelling Kit *Microscale Optimised for Labelling 50 µg Antibody Per Reaction*

Although FITC is still the most popular fluorescent labelling dye for preparing green fluorescent bioconjugates, there are certain limitations with FITC, such as severe photobleaching for microscope imaging and pH-sensitive fluorescence. Protein conjugates prepared with iFluor™ 488 dyes (Ex/Em=497 nm/525 nm) are far superior compared to conjugates of fluorescein derivatives such as FITC. iFluor™ 488 conjugates are significantly brighter than fluorescein conjugates and are much more photostable. Additionally, the fluorescence of iFluor™ 488 is not affected by pH (4-10). This pH insensitivity is a major improvement over fluorescein, which emits its maximum fluorescence only at pH above 9. ReadiLink™ labelling kits essentially only require 2 simple mixing steps without a column purification needed. iFluor™ 488 SE used in this ReadiLink™ kit is reasonably stable and shows good reactivity and selectivity with protein amino groups. The kit has all the essential components for labelling ~2×50 ug antibody. Each of the two vials of iFluor™ 488 dye provided in the kit is Optimised for labelling ~50 µg antibody. iFluor™ 488 SE protein labelling kit provides a convenient method to label monoclonal, polyclonal antibodies or other proteins (>10 kDa) with the iFluor™ 488 SE.

Detailed Information:   Protocol   References   MSDS   Spectrum

ReadiLink™ iFluor™ 555 Antibody Labelling Kit *Microscale Optimised for Labelling 50 µg Antibody Per Reaction*

iFluor™ 555 dyes have fluorescence excitation and emission maxima of ~550 nm and ~570 nm respectively. iFluor™ 555 family has the spectral properties essentially identical to those of Cy3® (Cy3® is the trademark of GE Healthcare). Compared to Cy3 probes iFluor™ 555 family has much stronger fluorescence and higher photostability. Their fluorescence is pH-independent from pH 3 to 11. These spectral characteristics make this new dye family a superior alternative to Cy3®. iFluor™ 555 family has become an excellent replacement for Cy3 and Alexa Fluor® 555 labelling dye (Alexa Fluor® is the trademark of Invitrogen). ReadiLink™ labelling kits essentially only require 2 simple mixing steps without a column purification needed. iFluor™ 555 SE used in this ReadiLink™ kit is reasonably stable and shows good reactivity and selectivity with protein amino groups. The kit has all the essential components for labelling ~2×50 ug antibody. Each of the two vials of iFluor™ 555 dye provided in the kit is Optimised for labelling ~50 µg antibody. iFluor™ 555 SE protein labelling kit provides a convenient method to label monoclonal, polyclonal antibodies or other proteins (>10 kDa) with the iFluor™ 555 SE.

Detailed Information:   Protocol   References   MSDS   Spectrum

ReadiLink™ iFluor™ 594 Antibody labelling Kit *Microscale Optimised for labelling 50 µg Antibody Per Reaction*

iFluor™ 594 dyes have fluorescence excitation and emission maxima of ~590 nm and ~610 nm respectively. iFluor™ 594 family has the spectral properties similar to those of Texas Red® and Alexa Fluor® 594 (Texas Red® and Alexa Fluor® 594 are the trademarks of Invitrogen). iFluor™ 594 family is pH-independent from pH 3 to 11. These spectral characteristics make this new dye family an excellent alternative to Texas Red® and Alexa Fluor® 594.Compared to Texas Red®, iFluor™ 594 is much easier to be conjugated with RPE with much higher conjugation yield, and the resulted RPE-iFluor™ 594 tandem has better FRET efficiency.ReadiLink™ labelling kits essentially only require 2 simple mixing steps without a column purification needed. iFluor™ 594 SE used in this ReadiLink™ kit is reasonably stable and shows good reactivity and selectivity with protein amino groups. The kit has all the essential components for labelling ~2×50 ug antibody. Each of the two vials of iFluor™ 594 dye provided in the kit is Optimised for labelling ~50 µg antibody. iFluor™ 594 SE protein labelling kit provides a convenient method to label monoclonal, polyclonal antibodies or other proteins (>10 kDa) with the iFluor™ 594 SE.

Detailed Information:   Protocol   References   MSDS   Spectrum

ReadiLink™ iFluor™ 633 Antibody labelling Kit *Microscale Optimised for labelling 50 µg Antibody Per Reaction*

iFluor™ 633 dyes have fluorescence excitation and emission maxima close to ~633 nm and ~655 nm respectively with good photostability. Our in-house comparable studies indicated that our iFluor™ 633 dyes are significantly brighter than the corresponding Cy5®. These spectral characteristics make them a superior alternative to Cy5® and Alexa Fluor®633 (Cy5® and Alexa Fluor® are the trademarks of GE Healthcare and Invitrogen respectively). iFluor™ 633 conjugates have been widely used in fluorescence animal imaging applications. ReadiLink™ labelling kits essentially only require 2 simple mixing steps without a column purification needed. iFluor™ 633 SE used in this ReadiLink™ kit is reasonably stable and shows good reactivity and selectivity with protein amino groups. The kit has all the essential components for labelling ~2×50 ug antibody. Each of the two vials of iFluor™ 633 dye provided in the kit is Optimised for labelling ~50 µg antibody. iFluor™ 633 SE protein labelling kit provides a convenient method to label monoclonal, polyclonal antibodies or other proteins (>10 kDa) with the iFluor™ 633 SE.

Detailed Information:   Protocol   References   MSDS   Spectrum

ReadiLink™ iFluor™ 647 Antibody labelling Kit *Microscale Optimised for labelling 50 µg Antibody Per Reaction*

iFluor™ 555 dyes have fluorescence excitation and emission maxima of ~550 nm and ~570 nm respectively. iFluor™ 647 family has the spectral properties essentially identical to those of Cy5® (Cy5® is the trademark of GE Healthcare). Compared to Cy5 probes iFluor™ 647 family has much stronger fluorescence and higher photostability. Their fluorescence is pH-independent from pH 3 to 11. These spectral characteristics make this new dye family a superior alternative to Cy5®. iFluor™ 647 family has become an excellent replacement for Cy5 and Alexa Fluor® 647 labelling dye (Alexa Fluor® is the trademark of Invitrogen). ReadiLink™ labelling kits essentially only require 2 simple mixing steps without a column purification needed. iFluor™ 647 SE used in this ReadiLink™ kit is reasonably stable and shows good reactivity and selectivity with protein amino groups. The kit has all the essential components for labelling ~2×50 ug antibody. Each of the two vials of iFluor™ 647 dye provided in the kit is Optimised for labelling ~50 µg antibody. iFluor™ 647 SE protein labelling kit provides a convenient method to label monoclonal, polyclonal antibodies or other proteins (>10 kDa) with the iFluor™ 647 SE.

Detailed Information:   Protocol   References   MSDS   Spectrum

ReadiLink™ iFluor™ 680 Antibody labelling Kit *Microscale Optimised for labelling 50 µg Antibody Per Reaction*

iFluor™ 680 dyes have fluorescence excitation and emission maxima close to 680 nm and 700 nm respectively with good photostability. These spectral characteristics make them an excellent alternative to Cy5.5®, IRDye® 700 and Alexa Fluor® 680 (Cy5.5®, IRDye® and Alexa Fluor® are the trademarks of GE Healthcare, Li-COR and Invitrogen respectively). ReadiLink™ labelling kits essentially only require 2 simple mixing steps without a column purification needed. iFluor™ 680 SE used in this ReadiLink™ kit is reasonably stable and shows good reactivity and selectivity with protein amino groups. The kit has all the essential components for labelling ~2×50 ug antibody. Each of the two vials of iFluor™ 680 dye provided in the kit is Optimised for labelling ~50 µg antibody. iFluor™ 680 SE protein labelling kit provides a convenient method to label monoclonal, polyclonal antibodies or other proteins (>10 kDa) with the iFluor™ 680 SE.

Detailed Information:   Protocol   References   MSDS   Spectrum

ReadiLink™ iFluor™ 700 Antibody labelling Kit *Microscale Optimised for labelling 50 µg Antibody Per Reaction*

iFluor™ 700 dyes have fluorescence excitation and emission maxima close to 690 nm and 710 nm respectively. These spectral characteristics make them an excellent alternative to Alexa Fluor® 700 (Alexa Fluor® is the trademark of Invitrogen). ReadiLink™ labelling kits essentially only require 2 simple mixing steps without a column purification needed. iFluor™ 700 SE used in this ReadiLink™ kit is reasonably stable and shows good reactivity and selectivity with protein amino groups. The kit has all the essential components for labelling ~2×50 ug antibody. Each of the two vials of iFluor™ 700 dye provided in the kit is Optimised for labelling ~50 µg antibody. iFluor™ 700 SE protein labelling kit provides a convenient method to label monoclonal, polyclonal antibodies or other proteins (>10 kDa) with the iFluor™ 700 SE.

Detailed Information:   Protocol   References   MSDS   Spectrum

ReadiLink™ iFluor™ 750 Antibody labelling Kit *Microscale Optimised for labelling 50 µg Antibody Per Reaction*

iFluor™ 750 dyes have fluorescence excitation and emission maxima close to 750 nm and 780 nm respectively with good photostability. Our in-house comparable studies indicated that our iFluor™ 750 dyes are significantly brighter than the corresponding Cy7® and Alexa Fluor® 750. These spectral characteristics make them a superior alternative to Cy7® and Alexa Fluor® 750 (Cy7® and Alexa Fluor® are the trademarks of GE Healthcare and Invitrogen respectively). iFluor™ 750 conjugates have been widely used in fluorescence animal imaging applications. ReadiLink™ labelling kits essentially only require 2 simple mixing steps without a column purification needed. iFluor™ 750 SE used in this ReadiLink™ kit is reasonably stable and shows good reactivity and selectivity with protein amino groups. The kit has all the essential components for labelling ~2×50 ug antibody. Each of the two vials of iFluor™ 750 dye provided in the kit is Optimised for labelling ~50 µg antibody. iFluor™ 750 SE protein labelling kit provides a convenient method to label monoclonal, polyclonal antibodies or other proteins (>10 kDa) with the iFluor™ 750 SE.

Detailed Information:   Protocol   References   MSDS   Spectrum

ReadiLink™ iFluor™ 790 Antibody labelling Kit *Microscale Optimised for labelling 50 µg Antibody Per Reaction*

iFluor™ 790 dyes have fluorescence excitation and emission maxima close to 790 nm and 810 nm respectively. These spectral characteristics make them an excellent alternative to IRDye® 800 (IRDye® is the trademark of Li-COR). Our in-house comparable studies indicated that our iFluor 790 dyes are significantly brighter than the corresponding IRDye® 800. iFluor™ 790 conjugates have been widely used in fluorescence animal imaging applications. ReadiLink™ labelling kits essentially only require 2 simple mixing steps without a column purification needed. iFluor™ 790 SE used in this ReadiLink™ kit is reasonably stable and shows good reactivity and selectivity with protein amino groups. The kit has all the essential components for labelling ~2×50 ug antibody. Each of the two vials of iFluor™ 790 dye provided in the kit is Optimised for labelling ~50 µg antibody. iFluor™ 790 SE protein labelling kit provides a convenient method to label monoclonal, polyclonal antibodies or other proteins (>10 kDa) with the iFluor™ 790 SE.

Detailed Information:   Protocol   References   MSDS   Spectrum