Immunohistochemistry

Immunohistochemistry (IHC) is a commonly used technique to monitor protein expression and localization in the context of tissue morphology. It uses antibodies to detect and analyze protein expression while maintaining the composition, cellular characteristics, and structure of native tissue. Chemical fixation locks molecular interactions within and between cells into place. Tissue samples may be embedded in paraffin wax or frozen before being cut into thin slices and mounted onto slides for analysis. Tissue collection, preservation, and fixation can vary greatly depending on the sample or the target of interest. The precise binding that occurs between an antibody and its epitope allows detection of highly specific amino acid sequences within a protein, targeting defined regions, domains, or cleavage products. Antibodies can also detect specific post-translational modifications (PTM) on a protein.

IHC is used for disease diagnosis, biological research, and in drug development. For example, using specific tumor markers, physicians use IHC to diagnose if a tumor is benign or malignant, to determine its stage and grade, and to identify the cell type and origin of a metastasis in order to find the site of the primary tumor. A variety of other non-neoplastic diseases and conditions are diagnosed using IHC as a primary tool or as a confirmatory procedure. In a research context, IHC can be used alone or in conjunction with other analytical techniques to study, for example, normal tissue and organ development, pathological processes, wound healing, cell death and repair, and many other fields.

Figure 1. Immunohistochemical detection of EpCAM in FFPE lung adenocarcinoma tissue. The tissue sections were incubated with poly-HRP conjugated Goat anti-Rabbit IgG and then developed with Stayright™ Purple (Left) or DAB (Right), respectively. Cells were also counterstained with hematoxylin. Stayright™ Purple generates an intense stain with high sensitivity and clear resolution similar as DAB.

Product Ordering Information

 

Table 1. IHC Chromogens For Product Ordering Information

Cat# Product Name Size
45900 ReadiUse™ Stayright™ Purple *HRP Chromogen Premixed with Hydrogen Peroxide* 5 mL
45905 Stayright™ Purple 5 mL

Table 2. iFluor™ dye Styramide™ Reagents For Product Ordering Information

Cat# Product Name Ex (nm) Em (nm) Size
45000 iFluor™ 350 Styramide *Superior Replacement for Alexa Fluor 350 tyramide* 345 442 100 Slides
45020 iFluor™ 488 Styramide *Superior Replacement for Alexa Fluor 488 tyramide* 491 514 100 Slides
45025 iFluor™ 546 Styramide *Superior Replacement for Alexa Fluor 546 tyramide* 541 557 100 Slides
45027 iFluor™ 555 Styramide *Superior Replacement for Alexa Fluor 555 tyramide* 552 567 100 Slides
45030 iFluor™ 568 Styramide *Superior Replacement for Alexa Fluor 568 tyramide* 568 587 100 Slides
45035 iFluor™ 594 Styramide *Superior Replacement for Alexa Fluor 594 tyramide* 592 619 100 Slides
45045 iFluor™ 647 Styramide *Superior Replacement for Alexa Fluor 647 tyramide* 649 665 100 Slides
45050 iFluor™ 680 Styramide *Superior Replacement for Alexa Fluor 680 tyramide* 676 695 100 Slides
45055 iFluor™ 700 Styramide *Superior Replacement for Alexa Fluor 700 tyramide* 685 710 100 Slides
45065 iFluor™ 750 Styramide *Superior Replacement for Alexa Fluor 750 tyramide* 749 775 100 Slides
45070 iFluor™ 790 Styramide *Superior Replacement for Alexa Fluor 790 tyramide* 782 811 100 Slides

Table 3. Hapten-labeled Styramide™ Reagents For Product Ordering Information

Cat# Product Name Size
45300 Biotin Styramide *Superior Replacement for Biotin Tyramide* 100 Slides
45305 DIG Styramide *Superior Replacement for DIG tyramide* 100 Slides
45310 DNP Styramide *Superior Replacement for DNP tyramide* 100 Slides

Table 4. iFluor™ PSA™ Imaging Kits with Goat Anti-Rabbit IgG For Product Ordering Information

Cat# Product Name Ex (nm) Em (nm) Size
45200 iFluor 350™ PSA™ Imaging Kit with Goat Anti-Rabbit IgG 345 442 100 Tests
45205 iFluor 488™ PSA™ Imaging Kit with Goat Anti-Rabbit IgG 491 514 100 Tests
45220 iFluor 555™ PSA™ Imaging Kit with Goat Anti-Rabbit IgG 552 567 100 Tests
45230 iFluor 594™ PSA™ Imaging Kit with Goat Anti-Rabbit IgG 592 619 100 Tests
45240 iFluor 647™ PSA™ Imaging Kit with Goat Anti-Rabbit IgG 649 665 100 Tests

Table 5. iFluor™ PSA™ Imaging Kits with Goat Goat Anti-Mouse IgG For Product Ordering Information

Cat# Product Name Ex (nm) Em (nm) Size
45250 iFluor 350™ PSA™ Imaging Kit with Goat Anti-Mouse IgG 345 442 100 Tests
45260 iFluor 488™ PSA™ Imaging Kit with Goat Anti-Mouse IgG 491 514 100 Tests
45270 iFluor 555™ PSA™ Imaging Kit with Goat Anti-Mouse IgG 552 567 100 Tests
45280 iFluor 594™ PSA™ Imaging Kit with Goat Anti-Mouse IgG 592 619 100 Tests
45290 iFluor 647™ PSA™ Imaging Kit with Goat Anti-Mouse IgG 649 665 100 Tests

Table 6. Tyramide Stand-alone Reagents For Product Ordering Information

Cat# Product Name Ex (nm) Em (nm) Size
45100 iFluor™ 488 tyramide 491 514 200 slides
45105 iFluor™ 555 Tyramide 552 567 200 Slides
45110 iFluor™ 647 Tyramide 649 665 200 Slides
11070 AF488 tyramide reagent 491 518 200 slides
11075 AF546 tyramide reagent 554 570 200 slides
11082 AF594 tyramide reagent 590 617 200 slides
11065 Cy3 tyramide 555 565 1 mg
11066 Cy5 tyramide 644 665 1 mg
11061 Azido-Cy5 tyramide 644 665 1 mg

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