Dyed Polystyrene

Our non-functionalized microspheres are suitable for coating with antibody or other large proteins via adsorption. See TechNote 204, Adsorption to Microspheres, for a general adsorption protocol. Unless noted otherwise, these visibly dyed microspheres are supplied as a 5% solids suspension (w/v) and are available in these standard amounts: 0.5g, 1.0g, 1.5g, and 5.0g.

References

• Henderson, K., & Stewart, J. (2002). Factors influencing the measurement of oestrone sulphate by dipstick particle capture immunoassay. Journal of immunological methods, 270(1), 77-84.(0.3, 0.5, and 0.8µm dyed blue PS)
• Henderson, K., & Stewart, J. (2000). A dipstick immunoassay to rapidly measure serum oestrone sulfate concentrations in horses. Reproduction, Fertility and Development, 12(4), 183-189.(0.31µm dyed blue PS)
• Campbell, K., Fodey, T., Flint, J., Danks, C., Danaher, M., O’Keeffe, M., … & Elliott, C. (2007). Development and validation of a lateral flow device for the detection of nicarbazin contamination in poultry feeds. Journal of agricultural and food chemistry, 55(6), 2497-2503.(0.43µm dyed blue PS)

Biomolecules may be covalently immobilized to carboxyl-functionalized microspheres. A general coupling protocol is provided within TechNote 205, Covalent Coupling. Our dyed carboxyl microspheres are impregnated with vibrant dyes for optimal visualization. See our color palette for a representation of our standard colors. Unless noted otherwise, they are supplied as a 5% solids suspension (w/v) and are available in four standard volumes: 0.5g, 1.0g, 1.5g, and 5.0g.

REFERENCES

• Terao, Y, Takeshita, K, Nishiyama, Y, Morishita, N, Matsumoto, T, & Morimatsu, F. (2015) Promising Nucleic Acid Lateral Flow Assay Plus PCR for Shiga Toxin–Producing Escherichia coli. Journ of food protection, 78(8), 1560-1568.
• Sheng, W, Li, S, Liu, Y, Wang, J, Zhang, Y, & Wang, S. (2017). Visual and rapid lateral flow immunochromatographic assay for enrofloxacin using dyed polymer microspheres and quantum dots. Microchimica Acta, 184(11), 4313-4321.(Dyed Black carboxyl microspheres)

At times, our inventory includes streptavidin-coated dyed microspheres supplied as ~1% solids (w/v) aqueous suspensions.

REFERENCES

• Li S, Liu Y, Wang Y, Wang M, Liu C, & Wang Y. (2019) Rapid detection of Brucella spp. and elimination of carryover using multiple cross displacement amplification coupled with nanoparticles-based lateral flow biosensor. Frontiers in Cellular and Infection Microbiology, 9, 78. (Streptavidin Crimson Red nanoparticles [CP01R] for binding of biotinylated Brucella spp. amplicons in lateral flow biosensor.)

VISIBLE DYE COLOR PALETTE

The following color palette is provided to serve as a general reference only. Actual product hue may vary due to differences in microsphere composition and size, as well as the concentration of the suspension. Dyed spheres may also be coated (e.g. streptavidin) on a custom basis. Our custom capabilities include color matching, custom color formulation, or use of the client’s hydrophobic dye.

FLUORESCENT DYE SPECTRA

Here is a listing of our standard fluorescent dye spectra. See our standard fluorescent microspheres.

FLOW CYTOMETRY FLUOROCHOMES

See our Fluorescent Reference Standards.