Magnetic Microspheres & Particles

Magnefy™ are ~1µm high-performance superparamagnetic microspheres. As high surface area / high surface titer microparticles with a rapid separation profile, Magnefy offer a performance-driven solid phase for magnetic particle-based applications, including SPRI-based total DNA isolation (COOH), and molecular- and immunoassays. Contact us to explore using Magnefy as your OEM magnetic SPRI bead.

REFERENCES

• Fisher S, Barry A, Abreu J, Minie B, Nolan J, Delorey T M, et al. (2011). A scalable, fully automated process for construction of sequence-ready human exome targeted capture libraries. Genome Biol; 12(1), R1.
• Elkin CJ, Richardson PM, Fourcade HM, Hammon NM, Pollard MJ, Predki PF, et al. (2001). High-throughput plasmid purification for capillary sequencing. Genome Res; 11(7), 1269-1274.

PROMAG^® HP

ProMag^® HP (High Performance) have been meticulously engineered for use in assay development. The highly optimized composition ensures lowest autosignal, particularly with respect to chemiluminescence and exposed iron. ProMag^® COOH are supplied as ~2.5% solids (w/v) aqueous suspensions. ProMag^® Streptavidin are supplied as ~1% solids (w/v) aqueous suspensions.

REFERENCES

• Vemulapati S, & Erickson D. (2018) HERMES: rapid blood-plasma separation at the point-of-need. Lab on a Chip 18(21), 3285-3292.(ProMag HP COOH conjugated to anti-RBC antibody)

PROMAG^®

ProMag^® are highly uniform polymer-based magnetic spheres available in diameters of 1µm and 3µm. A unique surface means low nonspecific binding in protein-based systems, and superior handling without the use of surfactant. These high-binding beads are suitable for use across a range of research and diagnostic applications, whether you’re working at laboratory scale or have the more stringent requirements of high throughput applications.

MAGNETIC PARTICLE SAMPLE PACKS

Our many magnetic particle products uniquely address a wide range of applications in the life sciences, including cell isolations, affinity purifications, immunoassays, molecular assays. For development efforts, our NEW Magnetic Particle Sample Packs allow you to test different particles to find which yield optimal performance characteristics in your specific system. See PDS 749 for details.

COMPEL™ are highly uniform superparamagnetic microspheres ideal for applications that demand uniform particle response, such as miniaturized bioassays and separations. COMPEL™ are comprised of iron oxide crystals dispersed in a polymer matrix, with a functional polymer overcoating for the encapsulation of magnetite and the introduction of reactive groups. As they are polymer-based, they retain the low density that is advantageous for biomolecular assays. Our initial offerings include 3µm, 6µm, and 8µm beads with measured CVs of ≤ 5% and high levels of COOH-functionalization. The carboxyl-modified undyed COMPEL are supplied as ~5% solids (w/w) and the fluorescent and streptavidin-coated versions of COMPEL are supplied at ~1% solids (w/v).

COMPEL™ COOH MODIFIED

FLUORESCENCE COMPEL™

COMPEL’s polymer matrix is conducive to dyeing, and fluorescent versions are available. For suspension array development, see QuantumPlex™. See also fluorescence spectra

 STREPTAVIDIN COATED COMPEL™

REFERENCES

• Diekmann, R., Wolfson, D. L., Spahn, C., Heilemann, M., Schüttpelz, M., & Huser, T. (2016). Nanoscopy of bacterial cells immobilized by holographic optical tweezers. Nature Communications, 7.

Both Classical (MC catalog codes) and Encapsulated (ME catalog codes) superparamagnetic microspheres are synthesized using a process that results in the distribution of iron oxide crystals throughout the polymer matrix. The surfaces of Encapsulated magnetic microspheres are expected to be free of iron oxide, while the Classical version will have some exposed iron oxide. COMPEL microspheres (UMC catalog codes) are highly uniform and spherical. Fluorescent magnetic microspheres are available as ~1% solids (w/v) aqueous suspensions in four standard volumes: 1mL, 5mL, 10mL, and 100mL. See the use of our microspheres for cell labeling and MRI tracking in TSD 0748.

REFERENCES

• Hill, J, Dick, A, Raman, J V,  Thompson. R , Yu. Z , Hinds, K ,…Lederman, R (2003) Serial Cardiac Magnetic Resonance Imaging of Injected Mesenchymal Stem Cells Circulation.108:1009-1014
• Shapiro E M, Sharer K, Skrtic S, and Koretsky, A. P. (2006) In vivo detection of single cells by MRI. Magn. Reson. Med., 55: 242–249
• Shapiro, E M, Medford-Davis, L N, Fahmy, TM, Dunbar, CE, Koretsky, AP (2007 Antibody-mediated cell labeling of peripheral T cells with micron-sized iron oxide particles (MPIOs) allows single cell detection by MRI. Contrast Media Mol Imaging, 2: 147–153
• Shapiro, E M, Gonzalez-Perez, O, García-Verdugo, JM, Alvarez-Buylla, A, Koretsky, AP (2006) Magnetic resonance imaging of the migration of neuronal precursors generated in the adult rodent brain. NeuroImage, 32(3), 1150–1157

BioMag and BioMag Plus are ~1.5µm high-performance superparamagnetic microparticles widely used in assays and for the efficient separation of cells and purification of proteins or other biomolecules. Their irregular morphology provides much greater surface area than similarly-sized spherical particles, resulting in high binding capacities and efficient capture of target with conservative use of particles. The high iron oxide content allows for rapid and efficient magnetic separations, even from difficult, e.g. highly viscous, samples. for more information see TSD 725.pdf, Coupling Procedure for Attaching Oligonucleotides to BioMag^® Carboxyl.

BIOMAG^® SUPERPARAMAGNETIC IRON OXIDE

BioMag^® Superparamagnetic Iron Oxide may be used for applications in which a plain iron oxide particle is required. Particles are ~10µm in size, and are non-functionalized.

MAGNETIC PARTICLE SAMPLE PACKS

Our many magnetic particle products uniquely address a wide range of applications in the life sciences, including cell isolations, affinity purifications, immunoassays, molecular assays. For development efforts, our NEW Magnetic Particle Sample Packs allow you to test different particles to find which yield optimal performance characteristics in your specific system. See PDS 749 for details.

BioMag^® magnetic particles with affinity binding proteins may be coated with the primary antibody or biotinylated molecule of interest through a straightforward incubation. They are also suitable for off-the-shelf use in applications, such as immunoprecipitation or PCR product clean-up.

BIOMAG^® STREPTAVIDIN / BIOTIN

MAGNETIC PARTICLE SAMPLE PACKS

Our many magnetic particle products uniquely address a wide range of applications in the life sciences, including cell isolations, affinity purifications, immunoassays, molecular assays. For development efforts, our NEW Magnetic Particle Sample Packs allow you to test different particles to find which yield optimal performance characteristics in your specific system. See PDS 749 for details.

BIOMAG^® PROTEINS A & G

BIOMAG^® SECONDARY AB

BioMag^® secondary antibody particles are used extensively in the capture of immune complexes for immunoprecipitation and antibody-labeled cells for indirect cell separation. Secondary antibody coatings also afford a convenient method for binding specific primary antibodies to BioMag^® particles.

BIOMAG^® LECTIN

Our ConA- and WGA coated BioMag^®Plus microparticles provide a convenient means for isolating glycoproteins from serum or cell lysate, and for investigating other lectin/glycan-mediated processes. The BioMag^®Plus magnetic particle format provides high surface area, and permits easy and efficient separations.

REFERENCES

• Yang, Ganglong, et al. Selective isolation and analysis of glycoprotein fractions and their glycomes from hepatocellular carcinoma sera. Proteomics 13.9 (2013): 1481-1498.
• Skene, Peter J., and Steven Henikoff. “CUT&RUN: Targeted in situ genome-wide profiling with high efficiency for low cell numbers.” bioRxiv (2017): 193219. (BP531, BioMagPlus Con A)

Our many magnetic particle products uniquely address a wide range of applications in the life sciences, including cell isolations, affinity purifications, immunoassays, molecular assays. For development efforts, our Magnetic Particle Sampler Packs allow you to test different particles to find which yield optimal performance characteristics in your specific system. We’ve taken our popular magnetic particles & packaged them together for your convenience offering, Magnefy™, BioMag^®Plus, ProMag^® 1 Series and ProMag^® HP 3 Series.

Meet the newest addition to our family of magnetic particles–Magnefy™ ~1µm carboxylated superparamagnetic microspheres. As high surface area / high surface titer microparticles with a rapid separation profile, Magnefy offer an additional performance-driven solid phase for magnetic particle-based assays and isolations, particularly SPRI-based total DNA isolation. (Available for carboxyl pack only.)

BioMag Plus are ~1.5µm high-performance superparamagnetic microparticles widely used in assays and for the efficient separation of cells and purification of proteins or other biomolecules. Their irregular morphology provides much greater surface area than similarly-sized spherical particles, resulting in high binding capacities and efficient capture of target with conservative use of particles. The high iron oxide content allows for rapid and efficient magnetic separations, even from difficult, e.g. highly viscous, samples.

Our ProMag 1 Series microspheres from our original ProMag line offer high surface area, fast and uniform separations and a hydrophilic surface. They have been used in magnetic particle assays, and for precision isolations.

Finally, our ProMag HP 3 Series microspheres have a highly optimized composition that offers superior handling and fast separation rates in addition to lowest autosignal, particularly with respect to chemiluminescence and exposed iron. They offer the most hydrophilic surface for low NSB and precise capture of target.