Silica Microspheres

SiO2 with natural hydroxyl or silanol (SiOH) surface groups for adsorption of nucleotides with low protein binding (see TechNote 104). Non-functionalized silica microspheres are available as ~10% solids (w/w) aqueous suspensions or in the dry form in these standard amounts: 0.5g, 1.0g, 1.5g, and 5.0g.

SILICA IN AQUEOUS SUSPENSION

DRY SILICA

REFERENCES

• Ferguson JA, Steemers FJ, Walt DR. (2000) High-density fiber-optic DNA random microsphere array. Anal Chem; 72(22):5618-5624.
• Lauer S, Goldstein B, Nolan RL, Nolan JP. (2002) Analysis of cholera toxin-ganglioside interactions by flow cytometry. Biochemistry; 41(6):1742-51.
• Steinberg G, Stromsborg K, Thomas L, Barker D, Zhao C. (2004) Strategies for covalent attachment of DNA to beads. Biopolymers; 73(5):597-605.
• B Sharma, P Bugga, L Madison, A Henry, M Blaber, N Greeneltch, … R. Van Duyne (2016) Bisboronic Acids for Selective, Physiologically Relevant Direct Glucose Sensing with Surface-Enhanced Raman Spectroscopy  Am Chem Soc, 138 (42), pp 13952–13959
• G Steinberg-Tatman,M Huynh, D Barker, C Zhao (2006) Synthetic Modification of Silica Beads That Allows for Sequential Attachment of Two Different Oligonucleotides Bioconjugate Chem 17(3), 841-848
• N Clack, K Salaita, J Groves (2008) Electrostatic readout of DNA microarrays with charged microspheres. Nat Biotech 26,825-830
• Lu Q, Liu H, Cao T (2013) Efficient Isolation of Bone Marrow Adipocyte Progenitors by Silica Microbeads Incubation. Stem Cells Dev. V22:18
• Khan Z, Poetter K, Park DJ.(2008) Enhanced solid phase PCR: mechanisms to increase priming by solid support primers. Anal Biochem. Apr 15;375(2):391-3.
• Marrec P, Doglioli M, Grégori G, Dugenne M, Della Penna A, Bhairy N, … Thyssen M (2017) Coupling physics and biogeochemistry thanks to high resolution observations of the phytoplankton community structure in the North-Western Mediterranean Sea Biogeosciences Discuss, https://doi.org/10.5194/bg-2017-343 (0.4µm – 7µm silica microspheres as FSC standards for phytoplankton on the CytoBuoy CytoSense)
• Wang, Z. (2016). Microsphere super-resolution imaging. Nanoscience, 3, 193-210. (4.7µm silica microspheres)
• Crites, T. J., Maddox, M., Padhan, K., Muller, J., Eigsti, C., & Varma, R. (2015). Supported lipid bilayer technology for the study of cellular interfaces. Current protocols in cell biology, 24-5. (lipid bilayers [aAPCs, artificial antigen-presenting cells] on 5µm silica microspheres)
• Zanoni, M., Habimana, O., Amadio, J., & Casey, E. (2016). Antifouling activity of enzyme‐functionalized silica nanobeads. Biotechnology and bioengineering, 113(3), 501-512.(500nm silica microspheres, functionalized & coated with Proteinase K for biofilm / biofouling control)
• Tolić-Nørrelykke, SF, Schäffer, E, Howard, J, Pavone, FS, Jülicher, F., & Flyvbjerg, H. (2006) Calibration of optical tweezers with positional detection in the back focal plane. Review of scientific instruments, 77(10), 103101.(SS04001, 1.54µm silica microspheres; PS03001, 0.523µm polystyrene microspheres)
• Bhebhe N, Williams PAC, Rosales-Guzmán C, Rodriguez-Fajardo V, Forbes A. (2018)A vector holographic optical trap. Sci.Rep. Vol. 8:#17387  (Holographic optical tweezers with arrays of HOPS beams demonstrated using 2µm silica microspheres)

SiO2 with carboxyl (COOH) surface groups for the covalent attachment of biomolecules (see TechNote 104). Carboxyl silica microspheres are sold in 1.0g amounts. Supplied dry, they are available in four nominal mean diameters: ~0.5µm, ~1.0µm, ~2.0µm, and ~5.0µm.

REFERENCES

• Wang L, Yang C, Tan W. (2004) Dual-luminophore-doped silica nanoparticles for multiplexed signaling. Nano Lett; 5(1):37-43.
• Lauer, SA, Nolan, JP (2002) Development and characterization of Ni-NTA-bearing microspheres. Cytometry, 48: 136–145.
• Yoo CE, Moon HS, Kim YJ, Park JM, Park D, Han KY,…Park WY (2015) Highly dense, optically inactive silica microbeads for the isolation and identification of circulating tumor cells. Biomaterials Jan;75:271-8.  (CTC isolation via anti-EpCAM coated silica microspheres)
• Litvinov, J, Moen, S. T, Koh, CY, & Singh, AK (2016). Centrifugal sedimentation immunoassays for multiplexed detection of enteric bacteria in ground water. Biomicrofluidics, 10(1), 014103.(5µm carboxyl silica microspheres)
• Litvinov J, Moen ST, Berry GJ, Loeffelholz M, Singh AK, & Koh CY. (2017). Centrifugal Microfluidic Platform for Rapid, Multiplexed Detection of TB and HIV Biomarkers in Whole Blood Samples. Journal of Bioengineering & Biomedical Science, 6(SAND-2015-11159J).(SC05001, 5µm Silica-COOH microspheres)
• Iswardy E, Tsai TC, Cheng IF, Ho TC, Perng GC, & Chang HC. (2017). A bead-based immunofluorescence-assay on a microfluidic dielectrophoresis platform for rapid dengue virus detection. Biosensors and Bioelectronics, 95, 174-180.(Anti-flavivirus antibody coupled to 1µm Silica COOH [Cat. SC04000] via PolyLink Coupling Kit [Cat. PL01N])

SiO2 with amine (NH2) surface groups for the covalent attachment of biomolecules (see TechNote 104). Amine silica microspheres are sold in 1.0g amounts. Supplied dry, they are available in these nominal mean diameters: ~0.5µm, ~1.0µm, and ~5.0µm.

SiO2 with a streptavidin-coated surface for the attachment of biomolecules (See also PDS 721.pdf). Silica streptavidin microspheres are sold in these standard amounts: 1mL, 2mL, 5mL, and 10mL. Available at 1% solids (w/v), in three nominal mean diameters: ~0.5µm, ~1.0µm, and ~5.0µm.