Epigenetics is one of the fastest-growing areas of academic and pharmaceutical research, with many projects focused on understanding the functions of histone post-translational modifications (PTMs) on chromatin. Indeed, manipulating or altering histone PTM interacting proteins, such as “readers” and/or chromatin modifying enzymes, is an attractive therapeutic strategy.
However, the precise PTM targets of many of these proteins is unknown. Notably, there are few reliable methods available to screen proteins against PTMs in a physiological, nucleosome context. These approaches also lack the broad substrate diversity and assay sensitivity needed to detect binding across a wide array of related / common PTMs.