Rabbit anti-PGP9.5 + Cy™2 Donkey anti-Rabbit (711-225-152 green). Basement membranes, Mouse anti-collagen IV + Cy™3 Donkey anti-Mouse (711-165-152 red). Keratinocytes and endothelium, Ulex-biotin+ Cy™5 Streptavidin (016-170-084 blue). Images McAdams et al