NEW! Alexa Fluor® 555 and 568 conjugates

Jackson ImmunoResearch introduces Alexa Fluor® 555 and 568 secondary antibody conjugates, expanding orange channel options for streamlined panel design. These dyes complement our existing Alexa Fluor® range and are available in diverse target specificities (Donkey/Goat hosts).With minimal cross-reactivity, they reduce cross-talk in multi-primary antibody workflows.

Dye Laser line (nm) Exc (nm) Em (nm) Extinc co-eff Quantum Yield (Φ) MW (Da) Applications
Alexa Fluor® 568 568 577 602 1.4 0.69 792 Microscopy, SRM, Flow Cytometry, Fluorisa, Fluorescent Western Blotting
Alexa Fluor® 555 488/532 552 572 1.4 0.1 1250 Microscopy, Flow Cytometry, Fluorisa, Fluorescent Western Blotting

Indirect four-color immunostaining of HEp-2 cells.

  • Cellular proliferation visualized in green, using Rabbit A-Ki-67 followed by Alexa Fluor® 488-conjugated Donkey Anti-Rabbit (H+L) (711-545-152-JIR).
  • Microtubules visualized in yellow, using Mouse anti-α-tubulin followed by AlexaFluor® 568 conjugated Donkey Anti-Mouse IgG (H+L) (715-575-150-JIR).
  • Cell-to-cell contact visualized in red using Goat Anti- E-Cadherin, followed by Alexa Fluor® 647-conjugated Donkey Anti-Goat IgG (H+L) (705-605-147-JIR).
  • Blue – DAPI Nuclear Stain.

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