Bovine Anti-Goat IgG (H+L) (min X Bov, Ck, GP, Sy Hms, Hrs, Hu, Ms, Rb, Rat Sr Prot)

Bovine serum albumin (BSA) and dry milk are used extensively for blocking non-specific binding of antibodies and for stabilizing antibodies and other proteins during freeze drying and in dilute solutions. However, dry milk and most commercial sources of BSA contain bovine IgG which interferes with the use of any anti-goat IgG (as well as anti-sheep IgG) secondary antibodies. When BSA or dry milk are used for blocking, any bovine IgG which binds to a tissue or substrate surface will be labeled by anti-goat IgG. This background labeling may lead to false interpretations or needless repetition of experiments. Similarly, BSA and dry milk in antibody diluents may form immune complexes between the bovine IgG and anti-goat IgG, thus lowering antibody titer and creating backgound from unbound bovine IgG remaining in the diluent. Also, bovine IgG bound to cells after they are cultured in media containing fetal calf serum may result in background labeling on cell surfaces and on Western blots of cell lysate proteins.