Crystallization of LEXSY-produced Helminth proteins

LEXSY (Leishmania Expression System) is a eukaryotic protein expression platform based on the S1-classified protozoan L. tarentolae. It combines the advantages of prokaryotic systems (simple handling, fast growth) with those of eukaryotic ones (efficient folding, post-translational modifications).

More than 20 crystal structures of proteins produced with LEXSY have been reported so far, among them human Cu/Zn Superoxide dismutase^[1], Channelrhodopsin 2^[2] or Legumain^[3].

Here LEXSY has been successfully employed by Ebner et al.^[4] to express an active dimeric chitinase from the gastrointestinal nematode Trichuris suis (Pig whipworm), which was suitable for in vitro and in vivo assays as well as for crystallization (Fig. 1).

Crystallization Stock Solutions in larger volumes are available to reproduce previously grown protein crystals.

Crystallization Stock Solution

• High concentrated solution of a precipitant, salt or buffer
• Chemicals are those used in our JBScreens
• Freshly made after receipt of order
• Available in 100 ml volume

Individual JBScreen Condition

• Any condition of a JBScreen with identical chemicals in identical concentrations, e.g. JBScreen JCSG++, condition E2 is 2 M Ammonium sulfate, 100 mM MES pH 6.5 and 200 mM Sodium chloride
• Freshly made after receipt of order
• Available in 10 ml or 100 ml volumes