DNA/cDNA Labeling

Fluorophores and haptens, are the most commonly used labels for the generation of non-radioactive DNA/cDNA probes. They are enzymatically introduced into DNA/cDNA via modified nucleotides that are incorporated as substitutes for their natural counterparts in either a one-step procedure (introduction of a fluorescent or hapten-modified nucleotide) or a two-step procedure (introduction of a reactive group carrying nucleotide and subsequent fluorescent or hapten labeling).

Label	Labeling Kits	Single Nucleotides
Fluorophore	Fluorescent PCR Labeling Kits Fluorescent Nick Translation Labeling Kits	Fluorescent Nucleotides
Hapten	Biotin & Digoxigenin PCR Labeling Kits Biotin & Digoxigenin Nick Translation Labeling Kits Biotin & Digoxigenin 3′-End Oligonucleotide Labeling Kits	Biotinylated Nucleotides Digoxigenin-/DNP-modified Nucleotides
Amine		Amine-modified Nucleotides
CLICK		CLICK-functionalized Nucleotides
LNA		LNA-functionalized Nucleotides → Nucleotides & Nucleosides
Nucleotide dependent	Enzyme Labeling Reagent Kits (w/o labeled nucleotide)

Non-radioactive Labeling of DNA and RNA

Hapten-modified Nucleotides for DNA/RNA Labelling

Table 1: Standard enzymatic procedures for the preparation of non-radioactive DNA/cDNA probes.

Template	Method	Labeled Probe	Labeling site	Enzyme
DNA	PCR	DNA	random	Thermophilic polymerase e.g. Taq Polymerase
	MDA-based WGA			Phi29 DNA Polymerase
	Nick Translation			DNAse I / DNA Polymerase I
	Primer Extension			Klenow Fragment 3’-> 5’ exo-, Taq Polymerase
	Primer Extension		3’ OH	Terminal Deoxynucleotidyl Transferase (TdT)
RNA	Reverse Transcription	cDNA	random	Reverse Transcriptase e.g. MMLV

Fluorescent DNA/cDNA Labeling

Fluorescent Nucleotides

Table 1: Enzymatic incorporation of fluorescent Nucleotides.

PCR: Polymerase chain reaction with Taq Polymerase; MDA-based WGA: Multiple displacement amplification (MDA)-based whole genome amplification (WGA) with Phi29 DNA Polymerase; NT: Nick Translation with DNAse I / DNA Polymerase I;
Primer Extension: Primer Extension with Klenow 3′-5′exo^–; 3′-End Labeling: Incorporation with Terminal deoxynucleotidyl Transferase (TdT); RT: Reverse Transcription with Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV RT)

DNA Labeling							cDNA Labeling
Emission color	Nucleotide	Cat. No.	PCR	NT	Primer Extension	3’ End Labeling	RT
dUTP
Blue	AMCA-6-dUTP	NU-803-AMCA	2	2	n/a	n/a	n/a
	DEAC-dUTP	NU-803-DEAC	–	1	n/a	n/a	n/a
	dUTP-ATTO-425	NU-803-425	1	1,2	1	1	n/a
	dUTP-XX-ATTO-425	NU-803-XX-425	1	1	n/a	n/a	n/a
Green	dUTP-XX-AF488	NU-803-XX-AF488	1	1	n/a	n/a	n/a
	dUTP-XX-ATTO-488	NU-803-XX-488	1,2	1,2	1,2	1,2	n/a
	Fluorescein-12-dUTP	NU-803-FAMX	1,2	1,2	1,2	1,2	n/a
	Rhodamine-12-dUTP	NU-803-RHOX	–	1,2	1	1,2	n/a
Yellow-Green	dUTP-XX-ATTO-532	NU-803-532	1	1	1	1	n/a
Yellow	dUTP-Cy3	NU-803-Cy3	1,2	1,2	1	1	2
	dUTP-XX-Cy3	NU-803-XX-CY3	1	1	1	n/a	1
	dUTP-XX-AF555	NU-803-XX-AF555	1	1	n/a	n/a	n/a
	dUTP-ATTO-550	NU-803-550	–	1	1	1	n/a
Orange	dUTP-Texas Red	NU-803-TXR	1,2	1,2	2	n/a	n/a
	dUTP-XX-Texas Red	NU-803-XX-TXR	1,2	1,2	2	n/a	n/a
	dUTP-XX-AF594	NU-803-XX-AF594	1,2	1,2	1	n/a	n/a
	dUTP-XX-594	NU-803-XX-594	1	1	1
Red	dUTP-Cy5	NU-803-CY5	1,2	1,2	1	n/a	2
	dUTP-XX-Cy5	NU-803-XX-CY5	1	1	1	n/a	1
	dUTP-ATTO-647N	NU-803-647N	–	1,2	1	1	n/a
	dUTP-ATTO-643	NU-803-643	1	1	1
	dUTP-XX-ATTO-643	NU-803-XX-643	1	1	1
	dUTP-XX-AF647	NU-803-XX-AF647	1	1	n/a	n/a	n/a
	dUTP-ATTO-655	NU-803-655	–	1	1	1	n/a
dCTP
Green	Fluorescein-12-dCTP	NU-809-FAMX	1	1	n/a	n/a	n/a
Green	Rhodamine-12-dCTP	NU-809-RHOX	–	1	n/a	n/a	n/a
Yellow	dCTP-Cy3	NU-809-Cy3	1	1	n/a	n/a	n/a
Yellow	dCTP-ATTO-550	NU-809-550	–	1	n/a	n/a	n/a
Orange	dCTP-Texas Red	NU-809-TXR	1	1	n/a	n/a	n/a
Red	dCTP-Cy5	NU-809-CY5	1	1	n/a	n/a	n/a
Red	dCTP-ATTO-647N	NU-809-647N	–	1	n/a	n/a	n/a

¹: in-house tested
²: external reference (see datasheet)
n/a: no data available
– : not applicable

Non-radioactive Labeling of DNA and RNA

Products & Ordering
AMCA-6-dUTP NU-803-AMCA Aminoallyl-dUTP – AMCA	DEAC-dUTP NU-803-DEAC Aminoallyl-dUTP – DEAC	Aminoallyl-dUTP-XX-ATTO-488 NU-803-XX-488
Aminoallyl-dUTP-ATTO-425 NU-803-425	Aminoallyl-dUTP-XX-Azdye488 NU-803-XX-AF488	Fluorescein-12-dUTP NU-803-FAMX Fluorescein-X-(5-aminoallyl)-dUTP
Aminoallyl-dUTP-XX-ATTO-425 NU-803-XX-425	Rhodamine-12-dUTP NU-803-RHOX Rhodamine-X-(5-aminoallyl)-dUTP Rho-green-X-dUTP, RhG-dUTP, R110-dUTP	Aminoallyl-dUTP-XX-Azdye594 NU-803-XX-AF594
Aminoallyl-dUTP-XX-ATTO-532 NU-803-XX-532	Aminoallyl-dUTP-ATTO-550 NU-803-550	Aminoallyl-dUTP-XX-ATTO-594 NU-803-XX-594
Aminoallyl-dUTP-Cy3 NU-803-CY3	Aminoallyl-dUTP-Texas Red NU-803-TXR	Aminoallyl-dUTP-Cy5 NU-803-CY5
Aminoallyl-dUTP-XX-Cy3 NU-803-XX-CY3	Aminoallyl-dUTP-XX-Texas Red NU-803-XX-TXR	Aminoallyl-dUTP-XX-Cy5 NU-803-XX-CY5
Aminoallyl-dUTP-XX-AF555 NU-803-XX-AF555	Aminoallyl-dUTP-XX-ATTO-643 NU-803-XX-643	Aminoallyl-dUTP-ATTO-647N NU-803-647N
Fluorescein-12-dCTP NU-809-FAMX Fluorescein-X-5-Propargylamino-dCTP	Aminoallyl-dUTP-XX-Azdye647 NU-803-XX-AF647	Aminoallyl-dUTP-ATTO-643 NU-803-643
Rhodamine-12-dCTP NU-809-RHOX Rhodamine-X-5-propargylamino-dCTP Rho-green-X-dCTP, RhG-dCTP, R110-dCTP	Aminoallyl-dUTP-ATTO-655 NU-803-655	5-Propargylamino-dCTP-Cy3 NU-809-CY3
5-Propargylamino-dCTP-Texas Red NU-809-TXR	5-Propargylamino-dCTP-Cy5 NU-809-CY5	5-Propargylamino-dCTP-ATTO-550 NU-809-550
5-Propargylamino-dCTP-ATTO-647N NU-809-647N

Fluorescent PCR Labeling Kits

Labeled DNA probes e.g. for (Fluorescence) in situ hybridization((F)ISH are routinely prepared via PCR if template amounts are limited or amplification of specific DNA fragments is required.

We offer High Fidelity PCR Labeling Kits …

… for both fluorescent (e.g Cy^®-, ATTO^TM– or AzDye-), Biotin and Digoxigenin labeling
… suitable for preparation of both short (>100 bp) and long DNA probes (up to 4 kbp tested) due to a High Fidelity Polymerase blend consisting of Taq polymerase and a proofreading enzyme.
… with convenient configurations for application-specific assay optimization such as single nucleotide format for optimization of labeled dUTP/dTTP ratio, positive control reaction (amplification of 500 bp fragment), testkits containing selected labeled dUTPs to find the optimal label

Fluorophore Selection Guide for PCR & Nick Translation Labeling Kits

Products & Ordering
HighFidelity Fluorescein PCR Labeling Kit APP-101-FAMX Preparation of Fluorescein-labeled DNA probes by PCR	HighFidelity ATTO488 PCR Labeling Kit APP-101-488 Preparation of ATTO488-labeled DNA probes by PCR	HighFidelity AzDye488 PCR Labeling Kit APP-101-AF488 Preparation of AzDye488-labeled DNA probes by PCR
HighFidelity GREEN PCR Labeling Testkit APP-101-GREEN Preparation of FAMX-, ATTO488- and AzDye488-labeled DNA probes by PCR	HighFidelity Cy3 PCR Labeling Kit APP-101-CY3 Preparation of Cy3-labeled DNA probes by PCR	HighFidelity AzDye555 PCR Labeling Kit APP-101-AF555 Preparation of AzDye555-labeled DNA probes by PCR
HighFidelity YELLOW PCR Labeling Testkit APP-101-YELLOW Preparation of Cy3- and AzDye555-labeled DNA probes by PCR	HighFidelity AzDye594 PCR Labeling Kit APP-101-AF594 Preparation of AzDye594-labeled DNA probes by PCR	HighFidelity ATTO594 PCR Labeling Kit APP-101-594 Preparation of ATTO594-labeled DNA probes by PCR
HighFidelity ORANGE PCR Labeling Testkit APP-101-ORANGE Preparation of AzDye594-, ATTO594- and TexasRed-labeled DNA probes by PCR	HighFidelity Cy5 PCR Labeling Kit APP-101-CY5 Preparation of Cy5-labeled DNA probes by PCR	HighFidelity AzDye647 PCR Labeling Kit APP-101-AF647 Preparation of AzDye647-labeled DNA probes by PCR
HighFidelity ATTO643 PCR Labeling Kit APP-101-643 Preparation of ATTO643-labeled DNA probes by PCR	HighFidelity RED PCR Labeling Testkit APP-101-RED Preparation of Cy5-, AzDye647- and ATTO643-labeled DNA probes by PCR

Fluorescent Nick Translation Labeling Kits

Nick translation is the method of choice for the generation of labeled DNA probes (100- 500 bp) from large templates such as genomic DNA, cosmids, BAC clones. It is based on the concerted activities of DNAse I (introduction of random single-strand breaks (“nicks”) into double-stranded DNA) and Polymerase I that incorporates labeled dUTPs instead of their natural counterpart dTTP at the “nicked” sites thus creating ideal in situ hybridization probes. There is no net DNA synthesis in contrast to PCR-based labeling approaches.

Biotin & Digoxigenin Nick Translation Labeling Kits are available as well.

Fluorophore Selection Guide for DNA & RNA Labeling Kits

Products & Ordering
Atto425 NT Labeling Kit PP-305-425 Blue-green fluorescent DNA labeling by nick translation	Fluorescein NT Labeling Kit PP-305-FAMX Green fluorescent DNA labeling by nick translation	AzDye488 NT Labeling Kit PP-305-AF488 Green fluorescent DNA labeling by nick translation
Atto488 NT Labeling Kit PP-305-488 Green fluorescent DNA labeling by nick translation	Cy3 NT Labeling Kit PP-305-CY3 Yellow fluorescent DNA labeling by nick translation	Atto550 NT Labeling Kit PP-305-550 Yellow fluorescent DNA labeling by nick translation
AzDye555 NT Labeling Kit PP-305-AF555 Yellow fluorescent DNA labeling by nick translation	TexasRed NT Labeling Kit PP-305-TXR orange DNA labeling by nick translation	AzDye594 NT Labeling Kit PP-305-AF594 Orange fluorescent DNA labeling by nick translation
Atto647N NT Labeling Kit PP-305-647N Red fluorescent DNA labeling by nick translation	AzDye647 NT Labeling Kit PP-305-AF647 Red fluorescent DNA labeling by nick translation	Cy5 NT Labeling Kit PP-305-CY5 Red fluorescent DNA labeling by nick translation
Atto680 NT Labeling Kit PP-305-680 IR-red fluorescent DNA labeling by nick translation

Hapten Labeling of DNA/cDNA

Biotinylated Nucleotides

Table 1: Enzymatic incorporation of Biotinylated Nucleotides.

PCR: Polymerase chain reaction with Taq Polymerase; NT: Nick Translation with DNAse I / DNA Polymerase I;
Primer Extension: Primer Extension with Klenow 3′-5′exo^–; 3′-End Labeling: Incorporation with Terminal deoxynucleotidyl Transferase (TdT); RT:: Reverse Transcription with Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV RT)

DNA Labeling						cDNA Labeling
Nucleotide	Cat. No.	PCR	NT	Primer Extension	3’ End Labeling	RT
dUTP
Biotin-11-dUTP C5 position	NU-803-BIOX	1,2	2	2	2	2
Biotin-16-dUTP C5 position	NU-803-BIO16	1,2	2	2	2	2
dCTP
Biotin-11-dCTP C5 position	NU-809-BIOX	1,2	2	2	2	2
Biotin-16-dCTP C5 position	NU-809-BIO16	1	2	2	2	2
Biotin-14-dCTP N4 position	NU-956-BIO14	–	2	2	n/a	n/a
dATP
Biotin-7-dATP N6 position	NU-835-BIO	–	2	2	–	n/a
Biotin-14-dATP N6 position	NU-835-BIO14	–	2	2	n/a	n/a
Desthiobiotin-7-dATP N6 position	NU-835-Desthiobio	–	n/a	2	n/a	n/a
Biotin-11-dATP 7-Deaza position	NU-1175-BIOX	1,2	2	2	n/a	2
dGTP
Biotin-11-dGTP 7-Deaza position	NU-1615-BIOX	n/a	n/a	n/a	n/a	n/a
ddUTP
Biotin-11-ddUTP C5 position	NU-1619-BIOX	–	–	–	2	–
ddATP
Biotin-11-ddATP 7-Deaza position	NU-1612-BIOX	–	–	–	2	–

¹: in-house tested
²: external reference (see datasheet)
n/a: no data available
– : not applicable

Non-radioactive Labeling of DNA and RNA

Hapten-modified Nucleotides for DNA/RNA Labelling

Products & Ordering
Biotin-11-dUTP NU-803-BIOX Biotin-X-(5-aminoallyl)-dUTP	Biotin-16-dUTP NU-803-BIO16 Biotin-16-(5-aminoallyl)-dUTP
Biotin-16-dCTP NU-809-BIO16 Biotin-16-Propargylamino-dCTP	Biotin-7-dATP NU-835-BIO N6-(6-Aminohexyl)-dATP – Biotin
Biotin-11-dATP NU-1175-BIOX	Biotin-11-ddUTP NU-1619-BIOX Biotin-X-5-Propargylamino-ddUTP
Biotin-14-dATP NU-835-BIO14	Biotin-11-ddATP NU-1612-BIOX
Biotin-14-dCTP NU-956-BIO14	Biotin-14-dCTP NU-956-BIO14
Biotin-11-dGTP NU-1615-BIOX	Biotin-11-dGTP NU-1615-BIOX
D-Desthiobiotin-7-dATP NU-835-Desthiobio N6-(6-Aminohexyl)-dATP – D-Desthiobiotin	Biotin-11-dCTP NU-809-BIOX Biotin-X-5-Propargylamino-dCTP

Digoxigenin-/DNP-modified Nucleotides

Table 1: Enzymatic incorporation of Digoxigenin (DIG)-/(DNP)-modified nucleotides.

DNA Labeling						cDNA Labeling
Nucleotide	Cat. No.	PCR	NT	Primer Extension	3’ End Labeling	RT
dUTP
Digoxigenin-11-dUTP	NU-803-DIGX	1,2	1,2	2	2	2
Aminoallyl-dUTP-DNP	NU-803-DNP	1	n/a	n/a	n/a	n/a
ddUTP
Digoxigenin-11-ddUTP	NU-1619-DIGX	–	–	–	2	–

Non-radioactive Labeling of DNA and RNA

Labeling of in situ hybridization DNA probes

Products & Ordering
DIG-11-dUTP NU-803-DIGX	Aminoallyl-dUTP-DNP NU-803-DNP Aminoallyl-dUTP – Dinitrophenol, DNP-11-dUTP	DIG-11-ddUTP NU-1619-DIGX

Biotin & Digoxigenin PCR Labeling Kits

Labeled DNA probes e.g. for in situ hybridization (ISH) are routinely prepared via PCR if template amounts are limited or amplification of specific DNA fragments is required.

We offer High Fidelity PCR Labeling Kits …

… for both Biotin & Digoxigenin and fluorescent labeling
… suitable for preparation of both short (>100 bp) and long DNA probes (up to 4 kbp tested) due to a High Fidelity Polymerase blend consisting of Taq polymerase and a proofreading enzyme.
… with convenient configurations for application-specific assay optimization such as single nucleotide format for optimization of labeled dUTP/dTTP ratio and a positive control reaction (amplification of 500 bp fragment).

The combination of (poly)-HRP-conjugated Biotin/Digoxigenin detection reagents with AzDye 488 -, AzDye546- or AzDye546 594 -labeled tyramide further increases detection sensitivity of up to 100-fold.

Products & Ordering
HighFidelity Biotin16 PCR Labeling Kit APP-101-BIO16 Preparation of Biotin16-labeled DNA probes by PCR	HighFidelity Digoxigenin PCR Labeling Kit APP-101-DIGX Preparation of Digoxigenin-labeled DNA probes by PCR
AzDye488 tyramide reagent RNT-012	AzDye546 tyramide reagent RNT-013
AzDye594 tyramide reagent RNT-014	AzDye488 Streptavidin Conjugate RNT-015 Streptavidin
AzDye594 Streptavidin Conjugate RNT-016 Streptavidin,

Biotin & Digoxigenin Nick Translation Labeling Kits

The combination of (poly)-HRP-conjugated Biotin/Digoxigenin detection reagents with AzDye 488 -, AzDye546- or AzDye594 -labeled tyramide further increases detection sensitivity of up to 100-fold.

Fluorescent Nick Translation Labeling Kits are available as well.

Products & Ordering
Biotin16 NT Labeling Kit PP-310-BIO16	AzDye488 tyramide reagent RNT-012
Digoxigenin NT Labeling Kit PP-310-DIGX	AzDye546 tyramide reagent RNT-013
AzDye594 tyramide reagent RNT-014	AzDye488 Streptavidin Conjugate RNT-015 Streptavidin
AzDye594 Streptavidin Conjugate RNT-016 Streptavidin

Biotin & Digoxigenin 3' End Oligonucleotide Labeling Kits

3’-End labeled oligonucleotides can be used as sequence-specific protein binding or nucleic acid hybridization probes e.g. for EMSA, Northern or Southern blots. Compared to internal, random labeled probes, the label is located at the 3‘-End only and less likely interferes with probe binding.

The combination of (poly)-HRP-conjugated Biotin/Digoxigenin detection reagents with AzDye488 -, AzDye546- or AzDye594 -labeled tyramide further increases detection sensitivity of up to 100-fold.

Figure 1 Terminal deoxynucleotidyl Transferase (TdT) template-independently transfers labeled nucleotides to the 3’-OH group of ssDNA/oligonucleotides. The number of nucleotide and thus label incorporation depends on the type of nucleotide ((dd)UTP) and type of label (n = 1 for labeled ddUTP, n = 1 – 3 (average) for labeled UTP, n > 1 for labeled dUTP (multiple label, tail length is highly nucleotide specific).

Labeling efficiency of BIO 3'-End Oligonucleotide Labeling Kit vs. competitor

Kit	Label	# of Label	Labeling principle similar to …
BIO 3‘-End Oligonucleotide Labeling Kit with Biotin-11-UTP	Biotin	1-3	Biotin 3‘-End DNA Labeling Kit (Pierce/Thermo Scientific)
DIG 3‘-End Oligonucleotide Labeling Kit with Digoxigenin-11-ddUTP	Digoxigenin	1	DIG Oligonucleotide 3‘ End Labeling Kit, 2nd generation (Roche)
3‘-End Oligonucleotide Labeling Reagent Kit	nucleotide dependent	nucleotide dependent

Products & Ordering
BIO 3′-End Oligonucleotide Labeling Kit with Biotin-11-UTP APP-001	AzDye546 tyramide reagent RNT-013
DIG 3′-End Oligonucleotide Labeling Kit with Digoxigenin-11-ddUTP APP-002	AzDye594 tyramide reagent RNT-014
3′-End Oligonucleotide Labeling Reagent Kit APP-003	AzDye488 Streptavidin Conjugate RNT-015 Streptavidin
AzDye488 tyramide reagent RNT-012	AzDye594 Streptavidin Conjugate RNT-016 Streptavidin

Click Chemistry-based DNA/cDNA Labeling

CLICK-functionalized Nucleotides for DNA Labeling

Table 1: Enzymatic incorporation of CLICK-functionalized nucleotides.

PCR: Polymerase chain reaction with Taq Polymerase (CLK-T09, NU-1705) or family B polymerases Pwo, Deep Vent exo^– and KOD XL (CLK-T05/-T06/-T07); NT: Nick Translation with DNAse I / DNA Polymerase I;
Primer Extension: Primer Extension with family B polymerases Pwo, Deep Vent exo^– and KOD XL; 3′-End Labeling: Incorporation with Terminal deoxynucleotidyl Transferase (TdT); RT:: Reverse Transcription with Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV RT)

Nucleotide	Cat. No.	PCR	NT	Primer Extension	3’ End Labeling	RT
dUTP
5-DBCO-PEG4-dUTP	CLK-048	1	n/a	n/a	n/a	n/a
5-Azidomethyl-dUTP	CLK-084	n/a	n/a	n/a	n/a	n/a
Azide-PEG4-Aminoallyl-dUTP	NU-1705	1	n/a	n/a	2	n/a
5-Ethynyl-dUTP	CLK-T07	1,2	n/a	2	n/a	n/a
C8-Alkyne-dUTP	CLK-T05	1,2	n/a	2	n/a	n/a
5-TCO-PEG4-dUTP	CLK-035	1	n/a	n/a	n/a	n/a
5-Vinyl-dUTP	CLK-068	1	n/a	n/a	n/a	n/a
dCTP
C8-Alkyne-dCTP	CLK-T06	1,2	n/a	2	2	n/a
5-DBCO-PEG4-dCTP	CLK-060	1	n/a	n/a	n/a	n/a
5-Azido-PEG4-dCTP	CLK-070	1	n/a	n/a	n/a	n/a
dATP
N6-Azidohexyl-dATP	CLK-NU-002	1	n/a	n/a	n/a	n/a

¹: in-house tested
²: external reference (see datasheet)
n/a: no data available
– : not applicable

Non-radioactive Labeling of DNA and RNA

Click Chemistry background information

Products & Ordering
5-DBCO-PEG4-dUTP CLK-048	5-Azidomethyl-dUTP CLK-084
Azide-PEG4-aminoallyl-dUTP NU-1705	5-Ethynyl-dUTP (5-EdUTP) CLK-T07
C8-Alkyne-dUTP CLK-T05	5-TCO-PEG4-dUTP CLK-035 5-trans-Cyclooctene-PEG4-dUTP
5-Vinyl-dUTP CLK-068	C8-Alkyne-dCTP CLK-T06
5-DBCO-PEG4-dCTP CLK-060	5-Azido-PEG4-dCTP CLK-070
N6-(6-Azido)hexyl-dATP CLK-NU-002

Amine Labeling of DNA/cDNA

Amine-modified Nucleotides for DNA Labeling

Table 1: Enzymatic incorporation of Amine-modified nucleotides.

PCR: Polymerase chain reaction with Taq Polymerase; NT: Nick Translation with DNAse I / DNA Polymerase I;
Primer Extension: Primer Extension with Klenow 3′-5’exo-; 3′-End Labeling: Incorporation with Terminal deoxynucleotidyl Transferase (TdT); RT: Reverse Transcription with Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV RT)

Nucleotide	Cat. No.	PCR	NT	Primer Extension	3’ End Labeling	RT
dUTP
5-Aminoallyl-dUTP	NU-803	1,2	2	2	2	2
dCTP
5-Propargylamino-dCTP	NU-809	1	n/a	n/a	n/a	n/a
dATP
N6-6-Aminohexyl-dATP N6 position	NU-835	-	2	n/a	n/a	n/a
7-Deaza-7-Propargylamino-dATP 7-Deaza position	NU-1611	2	n/a	n/a	n/a	n/a

¹: in-house tested
²: external reference (see datasheet)
n/a: no data available
– : not applicable

Non-radioactive Labeling of DNA and RNA

Labeling of in situ hybridization DNA probes

Products & Ordering
Aminoallyl-dUTP – Solution NU-803	5-Propargylamino-dCTP – Solid NU-809-lyo
Aminoallyl-dUTP – Solid NU-803-LYO	N6-(6-Aminohexyl)-dATP NU-835
5-Propargylamino-dUTP NU-998	7-Deaza-7-propargylamino-dATP NU-1611
5-Propargylamino-dCTP – Solution NU-809

LNA Labeling of DNA/cDNA

Products & Ordering

HighFidelity LNA PCR Labeling Kit APP-101-LNA Preparation of LNA-labeled DNA probes by PCR

Enzyme Labeling Reagent Kits for DNA/cDNA Labeling

3′-End Oligonucleotide Labeling Reagents

Products & Ordering

3′-End Oligonucleotide Labeling Reagent Kit APP-003

DNA/cDNA Labeling

Table 1: Standard enzymatic procedures for the preparation of non-radioactive DNA/cDNA probes.

Fluorescent DNA/cDNA Labeling

Table 1: Enzymatic incorporation of fluorescent Nucleotides.

Hapten Labeling of DNA/cDNA

Table 1: Enzymatic incorporation of Biotinylated Nucleotides.

Table 1: Enzymatic incorporation of Digoxigenin (DIG)-/(DNP)-modified nucleotides.

CLICK-functionalized Nucleotides for DNA Labeling

Table 1: Enzymatic incorporation of CLICK-functionalized nucleotides.

Amine-modified Nucleotides for DNA Labeling

Table 1: Enzymatic incorporation of Amine-modified nucleotides.

3′-End Oligonucleotide Labeling Reagents

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