The position of the modification within the triphosphate moiety that renders a triphosphate non-hydrolyzable exerts a strong influence on its binding affinity to a particular enzyme and its hydrolysis rate. These kits are designed to identify the most suitable non-hydrolyzable ATP or GTP derivative for your application.
Each Non-hydrolyzable Nucleotide Test Kit contains a set of 5 typical non-hydrolyzable Nucleoside-5′-triphosphate analogs.