Real-Time PCR / qPCR

Name Cat. No. Size
Direct qPCR ProbesMaster PCR-396S 2 x 1,25 ml (250 reactions x 20 μl)
Direct qPCR ProbesMaster PCR-396L 10 x 1,25 ml (1250 reactions x 20 μl)
Direct qPCR ProbesMaster highROX PCR-397S 2 x 1,25 ml (250 reactions x 20 μl)
Direct qPCR ProbesMaster highROX PCR-397L 10 x 1,25 ml (1250 reactions x 20 μl)

JBS qPCR Master Series allows quantitative real-time analysis of DNA samples based on detection of labeled DNA probes. Master Mixes contain all reagents required for qPCR (except template, primer and labeled fluorescent probe) in a premixed 2x concentrated solution, including hot-start polymerase. Its activity is blocked at ambient temperature and switched on automatically at the onset of initial denaturation to prevent extension of nonspecifically annealed primers and primer-dimer formation.

Both qPCR ProbesMaster mixes with and without UNG (Uracil-N-Glycosylase) are available in combination with low ROX and high ROX as reference dye for cycler-internal signal normalization. To facilitate pipetting into opaque plasticware a blue dyed version of the qPCR ProbesMaster Mixes is available. Mixes are recommended for use with Dual Labeled Fluorescent Probes, e.g. TaqMan®, Molecular Beacons or FRET probes.

Name Cat. No. Size
qPCR ProbesMaster PCR-360S 2 x 1,25 ml (250 reactions x 20 μl)
qPCR ProbesMaster PCR-360L 10 x 1,25 ml (1250 reactions x 20 μl)
qPCR ProbesMaster lowROX PCR-361S 2 x 1,25 ml (250 reactions x 20 μl)
qPCR ProbesMaster lowROX PCR-361L 10 x 1,25 ml (1250 reactions x 20 μl)
qPCR ProbesMaster highROX PCR-362S 2 x 1,25 ml (250 reactions x 20 μl)
qPCR ProbesMaster highROX PCR-362L 10 x 1,25 ml (1250 reactions x 20 μl)
qPCR ProbesMaster UNG PCR-363S 2 x 1,25 ml (250 reactions x 20 μl)
qPCR ProbesMaster UNG PCR-363L 10 x 1,25 ml (1250 reactions x 20 μl)
qPCR ProbesMaster UNG lowROX PCR-364S 2 x 1,25 ml (250 reactions x 20 μl)
qPCR ProbesMaster UNG lowROX PCR-364L 10 x 1,25 ml (1250 reactions x 20 μl)
qPCR ProbesMaster UNG highROX PCR-365S 2 x 1,25 ml (250 reactions x 20 μl)
qPCR ProbesMaster UNG highROX PCR-365L 10 x 1,25 ml (1250 reactions x 20 μl)

Lyophilisates – Preloaded and Stable at Room Temperature

Ready-to-Use Lyophilisates are delivered in PCR reaction tube strips or 96-well plates preloaded with a complete master mix in a dry, room temperature stable format. Facilitate handling with ready-to-use lyophilisates: no need for freezing, thawing or pipetting on ice and the few remaining pipetting steps minimize the risk of errors or contaminations.

Name Cat. No. Size
qPCR ProbesMaster Lyophilisate PCR-156S 192 reactions x 20 μl
qPCR ProbesMaster Lyophilisate PCR-156L 960 reactions x 20 μl
qPCR SybrMaster Lyophilisate PCR-173S 192 reactions x 20 μl
qPCR SybrMaster Lyophilisate PCR-173L 960 reactions x 20 μl

Development, optimization and scale-up of custom-specific lyophilisates

Freeze-dryed mixes are stable at ambient temperatures and significantly reduce contamination risk, turnaround time and total costs.

Lyophilisation service of Jena Bioscience at a glance

Optimization

  • adaptation of in-house lyophilisates to customized applications
  • modification of liquid custom-established assays for lyophilisation
  • optimization of the lyophilisation process to improve product functionality, stability and appearance

Lyophilisation

  • addition of custom-synthesized primers and internal/positive control DNA
  • up-scaling from small batch sizes to several thousand assay per batch
  • highly efficient cryo- and lyoprotectors with minimal influence on PCR available

Packaging

  • lyophilisation of PCR mixes in single PCR tubes, 8-well tube strips, 96-well plates or custom-specific formats
  • thermosealing into aluminium composite foil according to individual demands
  • custom labeling

 

Ready-to-Use Lyophilisates containing custom primers are custom made master mix lyophilisates comprising primers, polymerase, dNTPs and reaction buffer. Provide us with primer sequence and we deliver preloaded PCR tubes and plates ready-made for your special application. The only thing you still have to do is adding template DNA, filling up with water and starting the cycler!

The JBS qPCR SybrMaster Series is designed for quantitative real-time analysis of DNA samples. The mixes contain all reagents required for qPCR (except template and primers) in a premixed 2x concentrated ready-to-use solution. The mixes are recommended for routine PCR applications, high throughput PCR or genotyping and provide an improved specificity and sensitivity when amplifying low-copy-number targets or working with complex backgrounds.
The fluorescent DNA stain SYBR® Green intercalates into the amplification product during the PCR process and allows the direct quantification of target DNA without the need of sequence-specific labeled probes (i.g. TaqMan® Probes).

High Resolution Melting (HRM) analysis is a powerful tool for detection of mutations, polymorphisms and epigenetic differences in DNA samples. It is a fast and cost effective alternative to other genotyping technologies.

UNG (Uracil-N-Glycosylase) eliminates carry-over contamination of DNA from previous PCR reactions. An UNG treatment at the onset of thermal cycling removes uracil residues from dU-containing DNA and prevents it from serving as template.

ROX reference dye does not take part in the PCR reaction but allows to normalize for non-PCR related signal variation.

SYBR® Green Fluorescent DNA Stain is a superior DNA intercalator dye specially developed for DNA analysis applications including real-time PCR (qPCR). Upon binding to DNA, the non-fluorescent dye becomes highly fluorescent while showing no detectable inhibition to the PCR process. The dye is extremely stable, providing convenience during routine handling. SYBR® Green is in contrast to EvaGreen® not recommended for high-resolution melting curve analysis (HRM).

 

Name Cat. No. Size
qPCR SybrMaster PCR-372S 2 x 1,25 ml (2x conc.)
qPCR SybrMaster PCR-372L 10 x 1,25 ml (2x conc.)
qPCR SybrMaster lowROX PCR-373S 2 x 1,25 ml (2x conc.)
qPCR SybrMaster lowROX PCR-373L 10 x 1,25 ml (2x conc.)
qPCR SybrMaster highROX PCR-374S 2 x 1,25 ml (2x conc.)
qPCR SybrMaster highROX PCR-374L 10 x 1,25 ml (2x conc.)
qPCR SybrMaster UNG PCR-375S 2 x 1,25 ml (2x conc.)
qPCR SybrMaster UNG PCR-375L 10 x 1,25 ml (2x conc.)
qPCR SybrMaster UNG lowROX PCR-376S 2 x 1,25 ml (2x conc.)
qPCR SybrMaster UNG lowROX PCR-376L 10 x 1,25 ml (2x conc.)
qPCR SybrMaster UNG highROX PCR-377S 2 x 1,25 ml (2x conc.)
qPCR SybrMaster UNG highROX PCR-377L 10 x 1,25 ml (2x conc.)

EvaGreen® is a registered trademark and licensed for sale by Biotium, Inc., Hayward, CA, USA.
SYBR® is a registered trademark of Invitrogen Corporation, Carlsbad, California, USA.

JBS qPCR GreenMaster Series is designed for quantitative real-time analysis of DNA samples including High Resolution Melting (HRM) curve analysis. The mixes contain all reagents required for qPCR (except template and primers) in a premixed 2x concentrated ready-to-use solution. The mixes are recommended for routine PCR applications, high throughput PCR or genotyping and provide an improved specificity and sensitivity when amplifying low-copy-number targets or working with complex backgrounds.
The fluorescent DNA stain EvaGreen® intercalates into the amplification product during the PCR process and allows the direct quantification of target DNA without the need of sequence-specific labeled probes (i.g. TaqMan® Probes).

High Resolution Melting (HRM) analysis is a powerful tool for detection of mutations, polymorphisms and epigenetic differences in DNA samples. It is a fast and cost effective alternative to other genotyping technologies.

UNG (Uracil-N-Glycosylase) eliminates carry-over contamination of DNA from previous PCR reactions. An UNG treatment at the onset of thermal cycling removes uracil residues from dU-containing DNA and prevents it from serving as template.

ROX reference dye does not take part in the PCR reaction but allows to normalize for non-PCR related signal variation.

EvaGreen® Fluorescent DNA Stain is a DNA intercalator dye specially developed for DNA analysis applications including real-time PCR (qPCR) and high-resolution DNA melting curve analysis (HRM). Upon binding to DNA, the non-fluorescent dye becomes highly fluorescent while showing no detectable inhibition to the PCR process. The dye is extremely stable both thermally and hydrolytically, providing convenience during routine handling.

 

Name Cat. No. Size
qPCR GreenMaster highROX PCR-368S 2 x 1,25 ml (2x conc.)
qPCR GreenMaster highROX PCR-368L 10 x 1,25 ml (2x conc.)
qPCR GreenMaster UNG PCR-369S 2 x 1,25 ml (2x conc.)
qPCR GreenMaster UNG PCR-369L 10 x 1,25 ml (2x conc.)

EvaGreen® is a registered trademark and licensed for sale by Biotium, Inc., Hayward, CA, USA.
SYBR® is a registered trademark of Invitrogen Corporation, Carlsbad, California, USA.

The JBS qPCR Multiplex Master Series has been specially developed and optimized for simultaneous real-time analysis of >4 target sequences in a single tube. The system overcomes multiplex limitations of conventional qPCR probe mixes combining with an above-average robustness for a multitude of known PCR inhibitors and an excellent sensitivity for amplification of lowest template amounts.
The 2x concentrated master mixes contain all reagents required for qPCR (except template, primers and labeled fluorescent probes) including a highly processive antibody-inhibited hot-start polymerase and ultra-pure dNTPs.
The mixes provide an extremely stringent automatic hot-start allowing reaction set-up and temporary storage at room temperature prior to PCR.

qPCR Multiplex Master Mixes are available in combination with low ROX and high ROX as reference dye for cycler-internal signal normalization. All mixes are recommended for use with Dual Labeled Fluorescent Probes, e.g. TaqMan®, Molecular Beacons or FRET probes.

Name Cat. No. Size
qPCR MultiplexMaster PCR-321S 2 x 1,25 ml (2x conc.)
qPCR MultiplexMaster PCR-321L 10 x 1,25 ml (2x conc.)
qPCR MultiplexMaster lowROX PCR-322S 2 x 1,25 ml (2x conc.)
qPCR MultiplexMaster lowROX PCR-322L 10 x 1,25 ml (2x conc.)
qPCR MultiplexMaster highROX PCR-323S 2 x 1,25 ml (2x conc.)
qPCR MultiplexMaster highROX PCR-323L 10 x 1,25 ml (2x conc.)

Helpful tools for Real-Time PCR, to test the function or control the quality of qPCR polymerases and qPCR assay conditons.

 

Name Cat. No. Size
Thermolabile UNG (Uracil N-Glycosylase) PCR-353 200 units
SYBR® Green Fluorescent DNA Stain PCR-378 500 μl x 100 μM
EvaGreen® Fluorescent DNA Stain PCR-379 500 μl x 100 μM
ROX Reference Dye PCR-351 1 ml (25 μM)

EvaGreen® is a registered trademark and licensed for sale by Biotium, Inc., Hayward, CA, USA.
SYBR® is a registered trademark of Invitrogen Corporation, Carlsbad, California, USA.

Dual Labeled Fluorescent Probes are DNA oligonucleotides of 20-30 bp carrying a fluorophore (5′-end) and a quencher (3′-end). The labeled probe hybridizes sequence-specifically to its complementary section of the amplicon. During DNA extension of each PCR cycle, the fluorophore reporter is being cleaved and released. The resulting, detectable fluorescence signal is proportional to the amount of accumulated PCR product. All labeled probes are purified by HPLC and quality checked by MALDI-TOF. Select from Jena Bioscience’s extensive reporter/quencher repertoire or inquire for alternative combinations (info@stratech.co.uk).

BHQ-1 Quencher

Please select your desired Fluorescent Probe by clicking the basket and type the related sequence into the pop-up window. Prices include up to 40 bases per probe, HPLC purification and MALDI ToF quality check to provide probes of exceptional purity. For longer oligonucleotides or other reporter/quencher combinations please inquire.

A yield of 1 OD represents approx. 33 µg of single-stranded DNA with an equal distribution of the four bases. This corresponds to approx. 5 nmol (50 µl / 100 µM) of a DNA probe consisting of 20 bases.

Name Cat. No. Size
5’ ATTO-425 / 3’ BHQ-1® FP-414-05 5 to 9 nmol
5’ ATTO-425 / 3’ BHQ-1® FP-414-10 10 to 19 nmol
5’ ATTO-425 / 3’ BHQ-1® FP-414-20 20 to 29 nmol
5’ ATTO-425 / 3’ BHQ-1® FP-414-30 30 to 49 nmol
5’ ATTO-425 / 3’ BHQ-1® FP-414-50 50 to 70 nmol
5’ LC®Cyan500 / 3’ BHQ-1® FP-120-05 5 to 9 nmol
5’ LC®Cyan500 / 3’ BHQ-1® FP-120-10 10 to 19 nmol
5’ LC®Cyan500 / 3’ BHQ-1® FP-120-20 20 to 29 nmol
5’ LC®Cyan500 / 3’ BHQ-1® FP-120-30 30 to 49 nmol
5’ LC®Cyan500 / 3’ BHQ-1® FP-120-50 50 to 70 nmol
5’ 6-FAM / 3’ BHQ-1® FP-180-05 5 to 9 nmol
5’ 6-FAM / 3’ BHQ-1® FP-180-10 10 to 19 nmol
5’ 6-FAM / 3’ BHQ-1® FP-180-20 20 to 29 nmol
5’ 6-FAM / 3’ BHQ-1® FP-180-30 30 to 49 nmol
5’ 6-FAM / 3’ BHQ-1® FP-180-50 50 to 70 nmol
5’ Fluo / 3’ BHQ-1® FP-190-05 5 to 9 nmol
5’ Fluo / 3’ BHQ-1® FP-190-10 10 to 19 nmol
5’ Fluo / 3’ BHQ-1® FP-190-20 20 to 29 nmol
5’ Fluo / 3’ BHQ-1® FP-190-30 30 to 49 nmol
5’ Fluo / 3’ BHQ-1® FP-190-50 50 to 70 nmol
5’ FITC / 3’ BHQ-1® FP-185-05 5 to 9 nmol
5’ FITC / 3’ BHQ-1® FP-185-10 10 to 19 nmol
5’ FITC / 3’ BHQ-1® FP-185-20 20 to 29 nmol
5’ FITC / 3’ BHQ-1® FP-185-30 30 to 49 nmol
5’ FITC / 3’ BHQ-1® FP-185-50 50 to 70 nmol
5’ ATTO-495 / 3’ BHQ-1® FP-418-05 5 to 9 nmol
5’ ATTO-495 / 3’ BHQ-1® FP-418-10 10 to 19 nmol
5’ ATTO-495 / 3’ BHQ-1® FP-418-20 20 to 29 nmol
5’ ATTO-495 / 3’ BHQ-1® FP-418-30 30 to 49 nmol
5’ ATTO-495 / 3’ BHQ-1® FP-418-50 50 to 70 nmol
5’ TET / 3’ BHQ-1® FP-122-05 5 to 9 nmol
5’ TET / 3’ BHQ-1® FP-122-10 10 to 19 nmol
5’ TET / 3’ BHQ-1® FP-122-20 20 to 29 nmol
5’ TET / 3’ BHQ-1® FP-122-30 30 to 49 nmol
5’ TET / 3’ BHQ-1® FP-122-50 50 to 70 nmol
5’ ATTO-520 / 3’ BHQ-1® FP-410-05 5 to 9 nmol
5’ ATTO-520 / 3’ BHQ-1® FP-410-10 10 to 19 nmol
5’ ATTO-520 / 3’ BHQ-1® FP-410-20 20 to 29 nmol
5’ ATTO-520 / 3’ BHQ-1® FP-410-30 30 to 49 nmol
5’ ATTO-520 / 3’ BHQ-1® FP-410-50 50 to 70 nmol
5’ JOE / 3’ BHQ-1® FP-123-05 5 to 9 nmol
5’ JOE / 3’ BHQ-1® FP-123-10 10 to 19 nmol
5’ JOE / 3’ BHQ-1® FP-123-20 20 to 29 nmol
5’ JOE / 3’ BHQ-1® FP-123-30 30 to 49 nmol
5’ JOE / 3’ BHQ-1® FP-123-50 50 to 70 nmol
5’ Yakima Yellow / 3’ BHQ-1® FP-134-05 5 to 9 nmol
5’ Yakima Yellow / 3’ BHQ-1® FP-134-10 10 to 19 nmol
5’ Yakima Yellow / 3’ BHQ-1® FP-134-20 20 to 29 nmol
5’ Yakima Yellow / 3’ BHQ-1® FP-134-30 30 to 49 nmol
5’ Yakima Yellow / 3’ BHQ-1® FP-134-50 50 to 70 nmol
5’ HEX / 3’ BHQ-1® FP-124-05 5 to 9 nmol
5’ HEX / 3’ BHQ-1® FP-124-10 10 to 19 nmol
5’ HEX / 3’ BHQ-1® FP-124-20 20 to 29 nmol
5’ HEX / 3’ BHQ-1® FP-124-30 30 to 49 nmol
5’ HEX / 3’ BHQ-1® FP-124-50 50 to 70 nmol
5’ ATTO-Rho6G / 3’ BHQ-1® FP-404-05 5 to 9 nmol
5’ ATTO-Rho6G / 3’ BHQ-1® FP-404-10 10 to 19 nmol
5’ ATTO-Rho6G / 3’ BHQ-1® FP-404-20 20 to 29 nmol
5’ ATTO-Rho6G / 3’ BHQ-1® FP-404-30 30 to 49 nmol
5’ ATTO-Rho6G / 3’ BHQ-1® FP-404-50 50 to 70 nmol

BHQ-2 Quencher

Please select your desired Fluorescent Probe by clicking the basket and type the related sequence into the pop-up window. Prices include up to 40 bases per probe, HPLC purification and MALDI ToF quality check to provide probes of exceptional purity. For longer oligonucleotides or other reporter/quencher combinations please inquire.

A yield of 1 OD represents approx. 33 µg of single-stranded DNA with an equal distribution of the four bases. This corresponds to approx. 5 nmol (50 µl / 100 µM) of a DNA probe consisting of 20 bases.

Products & Ordering
5’ 6-FAM / 3’ BHQ-2® FP-181 5’ Cy3B / 3’ BHQ-2® FP-423 5’ DY-480XL / 3’ BHQ-2® FP-194
Ex: 495 nm / Em: 520 nm Ex: 559 nm / Em: 570 nm Ex: 500 nm / Em: 630 nm
5’ Fluo / 3’ BHQ-2® FP-191 5’ TAMRA / 3’ BHQ-2® FP-126 5’ LC®Red640 / 3’ BHQ-2® FP-135
Ex: 495 nm / Em: 520 nm Ex: 564 nm / Em: 579 nm Ex: 625 nm / Em: 640 nm
5’ FITC / 3’ BHQ-2® FP-186 5’ ROX / 3’ BHQ-2® FP-127 5’ ATTO-Rho14 / 3’ BHQ-2® FP-199
Ex: 490 nm / Em: 525 nm Ex: 576 nm / Em: 601 nm Ex: 625 nm / Em: 646 nm
5’ TET / 3’ BHQ-2® FP-164 5’ TexasRed / 3’ BHQ-2® FP-132 5’ Cy5 / 3’ BHQ-2® FP-136
Ex: 521 nm / Em: 536 nm Ex: 586 nm / Em: 610 nm Ex: 646 nm / Em: 662 nm
5’ HEX / 3’ BHQ-2® FP-133 5’ LC®Red610 / 3’ BHQ-2® FP-131 5’ Cy5.5 / 3’ BHQ-2® FP-137
Ex: 535 nm / Em: 556 nm Ex: 590 nm / Em: 610 nm Ex: 683 nm / Em: 705 nm
5’ ATTO-Rho6G / 3’ BHQ-2® FP-405 5’ ATTO-Rho13 / 3’ BHQ-2® FP-196 5’ IRD700 / 3’ BHQ-2® FP-138
Ex: 533 nm / Em: 557 nm Ex: 603 nm / Em: 627 nm Ex: 685 nm / Em: 705 nm
5’ Cy3 / 3’ BHQ-2® FP-125
Ex: 546 nm / Em: 563 nm

BHQ-3 Quencher

Please select your desired Fluorescent Probe by clicking the basket and type the related sequence into the pop-up window. Prices include up to 40 bases per probe, HPLC purification and MALDI ToF quality check to provide probes of exceptional purity. For longer oligonucleotides or other reporter/quencher combinations please inquire.

A yield of 1 OD represents approx. 33 µg of single-stranded DNA with an equal distribution of the four bases. This corresponds to approx. 5 nmol (50 µl / 100 µM) of a DNA probe consisting of 20 bases.

Products & Ordering
5’ ATTO-Rho13 / 3’ BHQ-3® FP-197 5’ ATTO-Rho14 / 3’ BHQ-3® FP-400 5’ Cy5.5 / 3’ BHQ-3® FP-142
Ex: 603 nm / Em: 627 nm Ex: 625 nm / Em: 646 nm Ex: 683 nm / Em: 705 nm
5’ LC®Red640 / 3’ BHQ-3® FP-144 5’ Cy5 / 3’ BHQ-3® FP-141 5’ IRD700 / 3’ BHQ-3® FP-143
Ex: 625 nm / Em: 640 nm Ex: 646 nm / Em: 662 nm Ex: 685 nm / Em: 705 nm

BBQ®-650 Quencher

Please select your desired Fluorescent Probe by clicking the basket and type the related sequence into the pop-up window. Prices include up to 40 bases per probe, HPLC purification and MALDI ToF quality check to provide probes of exceptional purity. For longer oligonucleotides or other reporter/quencher combinations please inquire.

A yield of 1 OD represents approx. 33 µg of single-stranded DNA with an equal distribution of the four bases. This corresponds to approx. 5 nmol (50 µl / 100 µM) of a DNA probe consisting of 20 bases.

Products & Ordering
5’ HEX / 3’ BBQ®-650 FP-169 5’ TexasRed / 3’ BBQ®-650 FP-173 5’ ATTO-Rho14 / 3’ BBQ®-650 FP-401
Ex: 535 nm / Em: 556 nm Ex: 586 nm / Em: 610 nm Ex: 625 nm / Em: 646 nm
5’ ATTO-Rho6G / 3’ BBQ®-650 FP-407 5’ LC®Red610 / 3’ BBQ®-650 FP-174 5’ Cy5 / 3’ BBQ®-650 FP-176
Ex: 533 nm / Em: 557 nm Ex: 590 nm / Em: 610 nm Ex: 646 nm / Em: 662 nm
5’ Cy3 / 3’ BBQ®-650 FP-170 5’ ATTO-Rho13 / 3’ BBQ®-650 FP-198 5’ Cy5.5 / 3’ BBQ®-650 FP-177
Ex: 546 nm / Em: 563 nm Ex: 603 nm / Em: 627 nm Ex: 683 nm / Em: 705 nm
5’ TAMRA / 3’ BBQ®-650 FP-171 5’ DY-480XL / 3’ BBQ®-650 FP-195 5’ IRD700 / 3’ BBQ®-650 FP-178
Ex: 564 nm / Em: 579 nm Ex: 500 nm / Em: 630 nm Ex: 685 nm / Em: 705 nm
5’ ROX / 3’ BBQ®-650 FP-172 5’ LC®Red640 / 3’ BBQ®-650 FP-175
Ex: 576 nm / Em: 601 nm Ex: 625 nm / Em: 640 nm

ECLIPSE Quencher

Please select your desired Fluorescent Probe by clicking the basket and type the related sequence into the pop-up window. Prices include up to 40 bases per probe, HPLC purification and MALDI ToF quality check to provide probes of exceptional purity. For longer oligonucleotides or other reporter/quencher combinations please inquire.

A yield of 1 OD represents approx. 33 µg of single-stranded DNA with an equal distribution of the four bases. This corresponds to approx. 5 nmol (50 µl / 100 µM) of a DNA probe consisting of 20 bases.

Products & Ordering
5’ ATTO-390 / 3’ ECLIPSE FP-422 5’ ATTO-495 / 3’ ECLIPSE FP-419 5’ ATTO-Rho6G / 3’ ECLIPSE FP-406
Ex: 390 nm / Em: 476 nm Ex: 498 nm / Em: 526 nm Ex: 533 nm / Em: 557 nm
5’ ATTO-425 / 3’ ECLIPSE FP-415 5’ TET / 3’ ECLIPSE FP-153 5’ Cy3 / 3’ ECLIPSE FP-162
Ex: 439 nm / Em: 485 nm Ex: 521 nm / Em: 536 nm Ex: 546 nm / Em: 563 nm
5’ LC®Cyan500 / 3’ ECLIPSE FP-151 5’ ATTO-520 / 3’ ECLIPSE FP-411 5’ TAMRA / 3’ ECLIPSE FP-157
Ex: 450 nm / Em: 500 nm Ex: 517 nm / Em: 538 nm Ex: 564 nm / Em: 579 nm
5’ 6-FAM / 3’ ECLIPSE FP-182 5’ JOE / 3’ ECLIPSE FP-154 5’ ROX / 3’ ECLIPSE FP-158
Ex: 495 nm / Em: 520 nm Ex: 522 nm / Em: 548 nm Ex: 576 nm / Em: 601 nm
5’ Fluo / 3’ ECLIPSE FP-192 5’ Yakima Yellow / 3’ ECLIPSE FP-155 5’ TEXAS RED / 3’ ECLIPSE FP-159
Ex: 495 nm / Em: 520 nm Ex: 530 nm / Em: 549 nm Ex: 586 nm / Em: 610 nm
5’ FITC / 3’ ECLIPSE FP-187 5’ HEX / 3’ ECLIPSE FP-156 5’ LC®Red610 / 3’ ECLIPSE FP-160
Ex: 490 nm / Em: 525 nm Ex: 535 nm / Em: 556 nm Ex: 590 nm / Em: 610 nm

DABCYL Quencher

Please select your desired Fluorescent Probe by clicking the basket and type the related sequence into the pop-up window. Prices include up to 40 bases per probe, HPLC purification and MALDI ToF quality check to provide probes of exceptional purity. For longer oligonucleotides or other reporter/quencher combinations please inquire.

A yield of 1 OD represents approx. 33 µg of single-stranded DNA with an equal distribution of the four bases. This corresponds to approx. 5 nmol (50 µl / 100 µM) of a DNA probe consisting of 20 bases.

Products & Ordering
5’ ATTO-390 / 3’ DABCYL FP-421 5’ FITC / 3’ DABCYL FP-188 5’ Yakima Yellow / 3’ DABCYL FP-117
Ex: 390 nm / Em: 476 nm Ex: 490 nm / Em: 525 nm Ex: 530 nm / Em: 549 nm
5’ ATTO-425 / 3’ DABCYL FP-413 5’ ATTO-495 / 3’ DABCYL FP-417 5’ HEX / 3’ DABCYL FP-115
Ex: 439 nm / Em: 485 nm Ex: 498 nm / Em: 526 nm Ex: 535 nm / Em: 556 nm
5’ LC®Cyan500 / 3’ DABCYL FP-111 5’ TET / 3’ DABCYL FP-113 5’ ATTO-Rho6G / 3’ DABCYL FP-403
Ex: 450 nm / Em: 500 nm Ex: 521 nm / Em: 536 nm Ex: 533 nm / Em: 557 nm
5’ 6-FAM / 3’ DABCYL FP-183 5’ ATTO-520 / 3’ DABCYL FP-409 5’ TAMRA / 3’ DABCYL FP-161
Ex: 495 nm / Em: 520 nm Ex: 517 nm / Em: 538 nm Ex: 564 nm / Em: 579 nm
5’ Fluo / 3’ DABCYL FP-193 5’ JOE / 3’ DABCYL FP-114
Ex: 495 nm / Em: 520 nm Ex: 522 nm / Em: 548 nm

TAMRA Quencher

Please select your desired Fluorescent Probe by clicking the basket and type the related sequence into the pop-up window. Prices include up to 40 bases per probe, HPLC purification and MALDI ToF quality check to provide probes of exceptional purity. For longer oligonucleotides or other reporter/quencher combinations please inquire.

A yield of 1 OD represents approx. 33 µg of single-stranded DNA with an equal distribution of the four bases. This corresponds to approx. 5 nmol (50 µl / 100 µM) of a DNA probe consisting of 20 bases.

Products & Ordering
5’ ATTO-390 / 3’ TAMRA FP-420 5’ ATTO-495 / 3’ TAMRA FP-416 5’ HEX / 3’ TAMRA FP-105
Ex: 390 nm / Em: 476 nm Ex: 498 nm / Em: 526 nm Ex: 535 nm / Em: 556 nm
5’ ATTO-425 / 3’ TAMRA FP-412 5’ TET / 3’ TAMRA FP-103 5’ ATTO-Rho6G / 3’ TAMRA FP-402
Ex: 439 nm / Em: 485 nm Ex: 521 nm / Em: 536 nm Ex: 533 nm / Em: 557 nm
5’ 6-FAM / 3’ TAMRA FP-179 5’ ATTO-520 / 3’ TAMRA FP-408 5’ ROX / 3’ TAMRA FP-168
Ex: 495 nm / Em: 520 nm Ex: 517 nm / Em: 538 nm Ex: 576 nm / Em: 601 nm
5’ Fluo / 3’ TAMRA FP-189 5’ JOE / 3’ TAMRA FP-104 5’ Cy5.5 / 3’ TAMRA FP-167
Ex: 495 nm / Em: 520 nm Ex: 522 nm / Em: 548 nm Ex: 683 nm / Em: 705 nm
5’ FITC / 3’ TAMRA FP-184 5’ Yakima Yellow / 3’ TAMRA FP-106 5’ IRD700 / 3’ TAMRA FP-166
Ex: 490 nm / Em: 525 nm Ex: 530 nm / Em: 549 nm Ex: 685 nm / Em: 705 nm

SUPPORT

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DELIVERY

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