| Products & Ordering | |
| SCRIPT Direct RT-qPCR ProbesMaster PCR-528 | SCRIPT Direct RT-qPCR ProbesMaster UNG highROX PCR-531 |
| Robust real-time RT-PCR master mix | Robust real-time RT-PCR master mix with UNG and ROX |
| for highly sensitive and specific amplification directly from tissue, swabs or whole blood | for highly sensitive and specific amplification directly from tissue, swabs or whole blood |
| SCRIPT Direct RT-qPCR ProbesMaster highROX PCR-529 | SCRIPT Direct RT-qPCR SybrMaster PCR-532 |
| Robust real-time RT-PCR master mix with ROX | Robust real-time RT-PCR master mix with SYBR Green |
| for highly sensitive and specific amplification directly from tissue, swabs or whole blood | for highly sensitive and specific amplification directly from tissue, swabs or whole blood |
| SCRIPT Direct RT-qPCR ProbesMaster UNG PCR-530 | SCRIPT Direct RT-qPCR SybrMaster highROX PCR-533 |
| Robust real-time RT-PCR master mix with UNG | Robust real-time RT-PCR master mix with SYBR Green and ROX |
| for highly sensitive and specific amplification directly from tissue, swabs or whole blood | for highly sensitive and specific amplification directly from tissue, swabs or whole blood |
Reverse Transcription / RT-PCR
SCRIPT One-Step RT-qPCR Master Mixes are designed for quantitative real-time analyses of RNA templates using Dual Labeled Fluorescent Probes or EvaGreen™ fluorescent DNA stain. All mixes are supplied as 2x concentrated solution containing all reagents required for RT-qPCR (except template and primers) for minimal pipetting steps and reduced contamination risk.
| Products & Ordering | |
| SCRIPT RT-qPCR ProbesMaster PCR-512 RT-real-time-PCR mix for using DNA probes 2 x conc. master mix, validated for COVID-19 testing | SCRIPT RT-qPCR ProbesMaster highROX PCR-513 RT-real-time-PCR mix for using DNA probes with highROX 2 x conc. master mix |
| SCRIPT RT-qPCR ProbesMaster UNG PCR-523 RT-real-time-PCR mix for using DNA probes 2 x conc. master mix, validated for COVID-19 testing | SCRIPT RT-qPCR ProbesMaster UNG highROX PCR-524 RT-real-time-PCR mix for using DNA probes with UNG and highROX 2 x conc. master mix |
Lyophilisates – Preloaded and Stable at Room Temperature
Ready-to-Use Lyophilisates are delivered in PCR reaction tube strips or 96-well plates preloaded with a complete master mix in a dry, room temperature stable format. The lyophilisates combine highest performance with convenience of use and stability. There is no need for freezing, thawing or pipetting on ice. The few remaining pipetting steps minimize the risk of errors or contaminations. Each vial contains enzyme, dNTPs and reaction buffer required for a 20 μl PCR assay. Simply fill up the vials with a premix of RNA template and primers and run the reverse transcripton in a thermocycler as usual.
Ready-to-Use Lyophilisates containing custom primers are custom made master mix lyophilisates comprising primers, enzyme, dNTPs and reaction buffer. Provide us with primer sequence and we deliver preloaded PCR tubes and plates ready-made for your special application. The only thing you still have to do is adding template RNA, filling up with water and starting the cycler!
Development, optimization and scale-up of custom-specific lyophilisates
Freeze-dryed mixes are stable at ambient temperatures and significantly reduce contamination risk, turnaround time and total costs.
Lyophilisation service of Jena Bioscience at a glance
Optimization
- adaptation of in-house lyophilisates to customized applications
- modification of liquid custom-established assays for lyophilisation
- optimization of the lyophilisation process to improve product functionality, stability and appearance
Lyophilisation
- addition of custom-synthesized primers and internal/positive control DNA
- up-scaling from small batch sizes to several thousand assay per batch
- highly efficient cryo- and lyoprotectors with minimal influence on PCR available
Packaging
- lyophilisation of PCR mixes in single PCR tubes, 8-well tube strips, 96-well plates or custom-specific formats
- thermosealing into aluminium composite foil according to individual demands
- custom labeling
Ready-to-Use Lyophilisates containing custom primers are custom made master mix lyophilisates comprising primers, polymerase, dNTPs and reaction buffer. Provide us with primer sequence and we deliver preloaded PCR tubes and plates ready-made for your special application. The only thing you still have to do is adding template DNA, filling up with water and starting the cycler!
| Products & Ordering | |
| SCRIPT RT-qPCR SybrMaster PCR-520 RT-real-time-PCR mix with SYBR® Green fluorescent DNA stain | SCRIPT RT-qPCR SybrMaster UNG PCR-526 RT-real-time-PCR mix with SYBR® Green fluorescent DNA stain and UNG |
| SCRIPT RT-qPCR SybrMaster highROX PCR-522 RT-real-time-PCR mix with SYBR® Green fluorescent DNA stain and highROX | SCRIPT RT-qPCR SybrMaster UNG highROX PCR-527 RT-real-time-PCR mix with SYBR® Green fluorescent DNA stain, UNG and highROX |
One-Step RT-PCR Kits are designed for performing highly sensitive and specific RT-PCR convenient in single tubes. The enzyme mix is based on a genetically engineered reverse transcriptase with enhanced thermal stability providing increased specificity, high cDNA yield and improved efficiency for highly structured and long cDNA fragments.
The kits contain all reagents required for RT-PCR (except template and primer) in one box to ensure fast and easy preparation with a minimum of pipetting steps.
| Products & Ordering | |
| SCRIPT RT-PCR Master (2x) PCR-525 One-Step RT-PCR Master Mix (2x conc.) for highly sensitive and specific amplification | SCRIPT High Fidelity RT-PCR Kit PCR-510 One-Step RT-PCR Kit for highly precise and fast amplification |
Reverse Transcriptase is a genetically engineered version of M-MLV Reverse Transcriptase (M-MLV RT) with eliminated RNase H activity and increased thermal stability. The enzyme is a RNA-directed DNA polymerase that synthesizes a complementary DNA strand initiating from a primer using single-stranded RNA or DNA as template. Its enhanced thermal stability in combination with the deactivated RNase H activity results in increased specificity, higher cDNA yield and improved efficiency for full length cDNA synthesis compared with standard M-MLV RT. The enzyme is recommended for synthesis of cDNA from 100 bp up to 10 kb length.
| Products & Ordering | |
| SCRIPT cDNA Synthesis Kit PCR-511 First strand cDNA synthesis with high sensitivity and efficiency | SCRIPT Reverse Transcriptase PCR-505 Reverse Transcriptase with increased thermal stability |
| Products & Ordering | ||
| RNase Inhibitor – recombinant PCR-392 Mus musculus, recombinant | SYBR® Green Fluorescent DNA Stain PCR-378 DNA intercalation dye for real-time PCR analysis | EvaGreen® Fluorescent DNA Stain PCR-379 DNA Intercalating dye for real-time qPCR fluorescence analysis including HRM |
| ROX Reference Dye PCR-351 Reference dye for fluorescence signal normalization | Thermolabile UNG (Uracil N-Glycosylase) PCR-353 Prevention of carry-over contaminations |
Dual Labeled Fluorescent Probes are DNA oligonucleotides of 20-30 bp carrying a fluorophore (5′-end) and a quencher (3′-end). The labeled probe hybridizes sequence-specifically to its complementary section of the amplicon. During DNA extension of each PCR cycle, the fluorophore reporter is being cleaved and released. The resulting, detectable fluorescence signal is proportional to the amount of accumulated PCR product. All labeled probes are purified by HPLC and quality checked by MALDI-TOF. Select from Jena Bioscience’s extensive reporter/quencher repertoire or inquire for alternative combinations (info@stratech.co.uk).
BHQ-1 Quencher
Please select your desired Fluorescent Probe by clicking the basket and type the related sequence into the pop-up window. Prices include up to 40 bases per probe, HPLC purification and MALDI ToF quality check to provide probes of exceptional purity. For longer oligonucleotides or other reporter/quencher combinations please inquire.
A yield of 1 OD represents approx. 33 µg of single-stranded DNA with an equal distribution of the four bases. This corresponds to approx. 5 nmol (50 µl / 100 µM) of a DNA probe consisting of 20 bases.
| Products & Ordering | ||
| 5’ ATTO-425 / 3’ BHQ-1® FP-414 Ex: 439 nm / Em: 485 nm | 5’ LC®Cyan500 / 3’ BHQ-1® FP-120 Ex: 450 nm / Em: 500 nm | 5’ 6-FAM / 3’ BHQ-1® FP-180 Ex: 495 nm / Em: 520 nm |
| 5’ Fluo / 3’ BHQ-1® FP-190 Ex: 495 nm / Em: 520 nm | 5’ FITC / 3’ BHQ-1® FP-185 Ex: 490 nm / Em: 525 nm | 5’ ATTO-495 / 3’ BHQ-1® FP-418 Ex: 498 nm / Em: 526 nm |
| 5’ TET / 3’ BHQ-1® FP-122 Ex: 521 nm / Em: 536 nm | 5’ ATTO-520 / 3’ BHQ-1® FP-410 Ex: 517 nm / Em: 538 nm | 5’ JOE / 3’ BHQ-1® FP-123 Ex: 522 nm / Em: 548 nm |
| 5’ Yakima Yellow / 3’ BHQ-1® FP-134 Ex: 530 nm / Em: 549 nm | 5’ HEX / 3’ BHQ-1® FP-124 Ex: 535 nm / Em: 556 nm | 5’ ATTO-Rho6G / 3’ BHQ-1® FP-404 Ex: 533 nm / Em: 557 nm |
BHQ-2 Quencher
Please select your desired Fluorescent Probe by clicking the basket and type the related sequence into the pop-up window. Prices include up to 40 bases per probe, HPLC purification and MALDI ToF quality check to provide probes of exceptional purity. For longer oligonucleotides or other reporter/quencher combinations please inquire.
A yield of 1 OD represents approx. 33 µg of single-stranded DNA with an equal distribution of the four bases. This corresponds to approx. 5 nmol (50 µl / 100 µM) of a DNA probe consisting of 20 bases.
| Products & Ordering | ||
| 5’ 6-FAM / 3’ BHQ-2® FP-181 | 5’ Cy3B / 3’ BHQ-2® FP-423 | 5’ DY-480XL / 3’ BHQ-2® FP-194 |
| Ex: 495 nm / Em: 520 nm | Ex: 559 nm / Em: 570 nm | Ex: 500 nm / Em: 630 nm |
| 5’ Fluo / 3’ BHQ-2® FP-191 | 5’ TAMRA / 3’ BHQ-2® FP-126 | 5’ LC®Red640 / 3’ BHQ-2® FP-135 |
| Ex: 495 nm / Em: 520 nm | Ex: 564 nm / Em: 579 nm | Ex: 625 nm / Em: 640 nm |
| 5’ FITC / 3’ BHQ-2® FP-186 | 5’ ROX / 3’ BHQ-2® FP-127 | 5’ ATTO-Rho14 / 3’ BHQ-2® FP-199 |
| Ex: 490 nm / Em: 525 nm | Ex: 576 nm / Em: 601 nm | Ex: 625 nm / Em: 646 nm |
| 5’ TET / 3’ BHQ-2® FP-164 | 5’ TexasRed / 3’ BHQ-2® FP-132 | 5’ Cy5 / 3’ BHQ-2® FP-136 |
| Ex: 521 nm / Em: 536 nm | Ex: 586 nm / Em: 610 nm | Ex: 646 nm / Em: 662 nm |
| 5’ HEX / 3’ BHQ-2® FP-133 | 5’ LC®Red610 / 3’ BHQ-2® FP-131 | 5’ Cy5.5 / 3’ BHQ-2® FP-137 |
| Ex: 535 nm / Em: 556 nm | Ex: 590 nm / Em: 610 nm | Ex: 683 nm / Em: 705 nm |
| 5’ ATTO-Rho6G / 3’ BHQ-2® FP-405 | 5’ ATTO-Rho13 / 3’ BHQ-2® FP-196 | 5’ IRD700 / 3’ BHQ-2® FP-138 |
| Ex: 533 nm / Em: 557 nm | Ex: 603 nm / Em: 627 nm | Ex: 685 nm / Em: 705 nm |
| 5’ Cy3 / 3’ BHQ-2® FP-125 | ||
| Ex: 546 nm / Em: 563 nm | ||
BHQ-3 Quencher
Please select your desired Fluorescent Probe by clicking the basket and type the related sequence into the pop-up window. Prices include up to 40 bases per probe, HPLC purification and MALDI ToF quality check to provide probes of exceptional purity. For longer oligonucleotides or other reporter/quencher combinations please inquire.
A yield of 1 OD represents approx. 33 µg of single-stranded DNA with an equal distribution of the four bases. This corresponds to approx. 5 nmol (50 µl / 100 µM) of a DNA probe consisting of 20 bases.
| Products & Ordering | ||
| 5’ ATTO-Rho13 / 3’ BHQ-3® FP-197 | 5’ ATTO-Rho14 / 3’ BHQ-3® FP-400 | 5’ Cy5.5 / 3’ BHQ-3® FP-142 |
| Ex: 603 nm / Em: 627 nm | Ex: 625 nm / Em: 646 nm | Ex: 683 nm / Em: 705 nm |
| 5’ LC®Red640 / 3’ BHQ-3® FP-144 | 5’ Cy5 / 3’ BHQ-3® FP-141 | 5’ IRD700 / 3’ BHQ-3® FP-143 |
| Ex: 625 nm / Em: 640 nm | Ex: 646 nm / Em: 662 nm | Ex: 685 nm / Em: 705 nm |
BBQ®-650 Quencher
Please select your desired Fluorescent Probe by clicking the basket and type the related sequence into the pop-up window. Prices include up to 40 bases per probe, HPLC purification and MALDI ToF quality check to provide probes of exceptional purity. For longer oligonucleotides or other reporter/quencher combinations please inquire.
A yield of 1 OD represents approx. 33 µg of single-stranded DNA with an equal distribution of the four bases. This corresponds to approx. 5 nmol (50 µl / 100 µM) of a DNA probe consisting of 20 bases.
| Products & Ordering | ||
| 5’ HEX / 3’ BBQ®-650 FP-169 | 5’ TexasRed / 3’ BBQ®-650 FP-173 | 5’ ATTO-Rho14 / 3’ BBQ®-650 FP-401 |
| Ex: 535 nm / Em: 556 nm | Ex: 586 nm / Em: 610 nm | Ex: 625 nm / Em: 646 nm |
| 5’ ATTO-Rho6G / 3’ BBQ®-650 FP-407 | 5’ LC®Red610 / 3’ BBQ®-650 FP-174 | 5’ Cy5 / 3’ BBQ®-650 FP-176 |
| Ex: 533 nm / Em: 557 nm | Ex: 590 nm / Em: 610 nm | Ex: 646 nm / Em: 662 nm |
| 5’ Cy3 / 3’ BBQ®-650 FP-170 | 5’ ATTO-Rho13 / 3’ BBQ®-650 FP-198 | 5’ Cy5.5 / 3’ BBQ®-650 FP-177 |
| Ex: 546 nm / Em: 563 nm | Ex: 603 nm / Em: 627 nm | Ex: 683 nm / Em: 705 nm |
| 5’ TAMRA / 3’ BBQ®-650 FP-171 | 5’ DY-480XL / 3’ BBQ®-650 FP-195 | 5’ IRD700 / 3’ BBQ®-650 FP-178 |
| Ex: 564 nm / Em: 579 nm | Ex: 500 nm / Em: 630 nm | Ex: 685 nm / Em: 705 nm |
| 5’ ROX / 3’ BBQ®-650 FP-172 | 5’ LC®Red640 / 3’ BBQ®-650 FP-175 | |
| Ex: 576 nm / Em: 601 nm | Ex: 625 nm / Em: 640 nm | |
ECLIPSE Quencher
Please select your desired Fluorescent Probe by clicking the basket and type the related sequence into the pop-up window. Prices include up to 40 bases per probe, HPLC purification and MALDI ToF quality check to provide probes of exceptional purity. For longer oligonucleotides or other reporter/quencher combinations please inquire.
A yield of 1 OD represents approx. 33 µg of single-stranded DNA with an equal distribution of the four bases. This corresponds to approx. 5 nmol (50 µl / 100 µM) of a DNA probe consisting of 20 bases.
| Products & Ordering | ||
| 5’ ATTO-390 / 3’ ECLIPSE FP-422 | 5’ ATTO-495 / 3’ ECLIPSE FP-419 | 5’ ATTO-Rho6G / 3’ ECLIPSE FP-406 |
| Ex: 390 nm / Em: 476 nm | Ex: 498 nm / Em: 526 nm | Ex: 533 nm / Em: 557 nm |
| 5’ ATTO-425 / 3’ ECLIPSE FP-415 | 5’ TET / 3’ ECLIPSE FP-153 | 5’ Cy3 / 3’ ECLIPSE FP-162 |
| Ex: 439 nm / Em: 485 nm | Ex: 521 nm / Em: 536 nm | Ex: 546 nm / Em: 563 nm |
| 5’ LC®Cyan500 / 3’ ECLIPSE FP-151 | 5’ ATTO-520 / 3’ ECLIPSE FP-411 | 5’ TAMRA / 3’ ECLIPSE FP-157 |
| Ex: 450 nm / Em: 500 nm | Ex: 517 nm / Em: 538 nm | Ex: 564 nm / Em: 579 nm |
| 5’ 6-FAM / 3’ ECLIPSE FP-182 | 5’ JOE / 3’ ECLIPSE FP-154 | 5’ ROX / 3’ ECLIPSE FP-158 |
| Ex: 495 nm / Em: 520 nm | Ex: 522 nm / Em: 548 nm | Ex: 576 nm / Em: 601 nm |
| 5’ Fluo / 3’ ECLIPSE FP-192 | 5’ Yakima Yellow / 3’ ECLIPSE FP-155 | 5’ TEXAS RED / 3’ ECLIPSE FP-159 |
| Ex: 495 nm / Em: 520 nm | Ex: 530 nm / Em: 549 nm | Ex: 586 nm / Em: 610 nm |
| 5’ FITC / 3’ ECLIPSE FP-187 | 5’ HEX / 3’ ECLIPSE FP-156 | 5’ LC®Red610 / 3’ ECLIPSE FP-160 |
| Ex: 490 nm / Em: 525 nm | Ex: 535 nm / Em: 556 nm | Ex: 590 nm / Em: 610 nm |
DABCYL Quencher
Please select your desired Fluorescent Probe by clicking the basket and type the related sequence into the pop-up window. Prices include up to 40 bases per probe, HPLC purification and MALDI ToF quality check to provide probes of exceptional purity. For longer oligonucleotides or other reporter/quencher combinations please inquire.
A yield of 1 OD represents approx. 33 µg of single-stranded DNA with an equal distribution of the four bases. This corresponds to approx. 5 nmol (50 µl / 100 µM) of a DNA probe consisting of 20 bases.
| Products & Ordering | ||
| 5’ ATTO-390 / 3’ DABCYL FP-421 | 5’ FITC / 3’ DABCYL FP-188 | 5’ Yakima Yellow / 3’ DABCYL FP-117 |
| Ex: 390 nm / Em: 476 nm | Ex: 490 nm / Em: 525 nm | Ex: 530 nm / Em: 549 nm |
| 5’ ATTO-425 / 3’ DABCYL FP-413 | 5’ ATTO-495 / 3’ DABCYL FP-417 | 5’ HEX / 3’ DABCYL FP-115 |
| Ex: 439 nm / Em: 485 nm | Ex: 498 nm / Em: 526 nm | Ex: 535 nm / Em: 556 nm |
| 5’ LC®Cyan500 / 3’ DABCYL FP-111 | 5’ TET / 3’ DABCYL FP-113 | 5’ ATTO-Rho6G / 3’ DABCYL FP-403 |
| Ex: 450 nm / Em: 500 nm | Ex: 521 nm / Em: 536 nm | Ex: 533 nm / Em: 557 nm |
| 5’ 6-FAM / 3’ DABCYL FP-183 | 5’ ATTO-520 / 3’ DABCYL FP-409 | 5’ TAMRA / 3’ DABCYL FP-161 |
| Ex: 495 nm / Em: 520 nm | Ex: 517 nm / Em: 538 nm | Ex: 564 nm / Em: 579 nm |
| 5’ Fluo / 3’ DABCYL FP-193 | 5’ JOE / 3’ DABCYL FP-114 | |
| Ex: 495 nm / Em: 520 nm | Ex: 522 nm / Em: 548 nm | |
TAMRA Quencher
Please select your desired Fluorescent Probe by clicking the basket and type the related sequence into the pop-up window. Prices include up to 40 bases per probe, HPLC purification and MALDI ToF quality check to provide probes of exceptional purity. For longer oligonucleotides or other reporter/quencher combinations please inquire.
A yield of 1 OD represents approx. 33 µg of single-stranded DNA with an equal distribution of the four bases. This corresponds to approx. 5 nmol (50 µl / 100 µM) of a DNA probe consisting of 20 bases.
| Products & Ordering | ||
| 5’ ATTO-390 / 3’ TAMRA FP-420 | 5’ ATTO-495 / 3’ TAMRA FP-416 | 5’ HEX / 3’ TAMRA FP-105 |
| Ex: 390 nm / Em: 476 nm | Ex: 498 nm / Em: 526 nm | Ex: 535 nm / Em: 556 nm |
| 5’ ATTO-425 / 3’ TAMRA FP-412 | 5’ TET / 3’ TAMRA FP-103 | 5’ ATTO-Rho6G / 3’ TAMRA FP-402 |
| Ex: 439 nm / Em: 485 nm | Ex: 521 nm / Em: 536 nm | Ex: 533 nm / Em: 557 nm |
| 5’ 6-FAM / 3’ TAMRA FP-179 | 5’ ATTO-520 / 3’ TAMRA FP-408 | 5’ ROX / 3’ TAMRA FP-168 |
| Ex: 495 nm / Em: 520 nm | Ex: 517 nm / Em: 538 nm | Ex: 576 nm / Em: 601 nm |
| 5’ Fluo / 3’ TAMRA FP-189 | 5’ JOE / 3’ TAMRA FP-104 | 5’ Cy5.5 / 3’ TAMRA FP-167 |
| Ex: 495 nm / Em: 520 nm | Ex: 522 nm / Em: 548 nm | Ex: 683 nm / Em: 705 nm |
| 5’ FITC / 3’ TAMRA FP-184 | 5’ Yakima Yellow / 3’ TAMRA FP-106 | 5’ IRD700 / 3’ TAMRA FP-166 |
| Ex: 490 nm / Em: 525 nm | Ex: 530 nm / Em: 549 nm | Ex: 685 nm / Em: 705 nm |
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