In the assay, the target miRNA molecule is hybridized with two oligos to form a RNA/DNA duplex. When the sequences are perfectly matched, they are aligned with the miRNA and the joint can be ligated with DNA ligase. A single nucleotide difference among miRNAs will block either the hybridization or the ligation. After the pair of oligos is ligated, the ligated molecules are subjected to real-time PCR analysis.
Benefits
- Convenient – All reagents, from cell lysis to PCR, are included in one kit.
- No RNA preparation – Cell lysates can be used directly in reverse transcription, although total RNA is perfectly fine.
- Real-time – Analysis can be monitored in real time.
miRNA Real-Time PCR Assay Kit
miRNAs are known to involve in multiple biological functions and abnormal expression is associated to human diseases such as cancers. They represent a promising new class of cancer biomarkers. A number of PCR methods have been developed for analyzing the expression level of miRNA. Based on its proprietary technology, Signosis has developed a highly sensitive and discriminative real-time PCR assay method for measuring miRNA expression. It implements oligo-ligation and SYBR green based real-time PCR. The assay can be used for quantitative analysis of miRNA expression in either total RNA or cell lysate without cDNA conversion.
miR-17-92 Cluster Real-Time PCR Assay Kit
The miR-17-92 cluster encoding miR-17-3p, miR-17-5p, miR-18a, miR-19a, miR-19b, miR–20a and miR-92 is highly expressed in tumor cells and are known to act as oncogenes. Expression of these miRNAs promotes cell proliferation, suppresses apoptosis of cancer cells, and induces tumor angiogenesis. New work reveals essential functions for these miRNAs not only in tumor formation but also during normal development of the heart, lungs, and immune system. A number of PCR methods have been developed for analyzing the expression level of miRNA. Based on its proprietary technology, Signosis has developed a highly sensitive and discriminative real-time PCR assay method for measuring multiple miRNA expression simultaneously, including miR-17-3P, miR-17-5p, miR-18a, miR-19a, miR-19b, miR-20a, miR-92. It implements oligo-ligation and SYBR green based real-time PCR. The assay can be used for quantitative analysis of miRNA expression in either total RNA or cell lysate without cDNA conversion.
Let7 Isoform Real-Time PCR
Let7 family is known to regulate proliferation and differentiation processes in species ranging from C- elegans to human. The mature forms (designated let-7a through let-7i ) differ at one to four positions from the let-7a sequence. The expression level of the isoforms is variable, which may play a diverse role in organ development and disease etiology. To distinguish these isoforms will help to elicit the mechanism of Let7 function. Based on its proprietary technology, Signosis has developed a highly sensitive and discriminative real-time PCR assay method for measuring miRNA expression of let7 isoforms (Let7a, Let7b, Let7c, Let7d, Let7e, Let7f, Let7g and Let7i) simultaneously. The assay implements oligo-ligation and SYBR green based real-time PCR, which can be used for quantitative analysis of miRNA expression in either total RNA or cell lysate without cDNA conversion.
