Immunodetection for pan influenza np antigens

Influenza viral nucleoprotein (NP) is a structural protein that plays a critical role in virus replication and host adaptation. Antibodies targeting NP proteins are commonly used for immunodetection of influenza viruses in various assays, including ELISA, lateral flow assay (LFA), and direct fluorescent antibody tests. These assays can be used in both research and clinical diagnostics. Due to the high frequency of antigenic drift or shift among different influenza strains, broad-spectrum influenza antibodies are particularly desired for flu diagnostics.

Sino Biological has identified six Pan antibody pairs against the NP protein of Influenza A and Influenza B, respectively. These matched pairs have been validated in the sandwich ELISA assay with high sensitivity (pg level). They also demonstrate broad reactivity against a large cohort of flu strains.

Antibody Pairs for Pan Influenza A NP Detection

Antibody Pairs for Pan Influenza B NP Detection

High Sensitivity (pg level)

Broad Spectrum and High Specificity

These antibody pairs can detect a broad spectrum of strains within the target subtype, without any cross-reactivity with the other subtypes.

24 kinds of influenza antigens from A or B have been tested by ELISA assay

The Aforementioned Antigens: 

High Binding Affinity

The binding affinity between immobilized Influenza A NP protein (Cat#: 40774-V08B) with the anti-NP antibody (Cat#: 40205-R063) in BLI assay.

Other Pan Influenza NP Antibodies

16 Pan Influenza A or B Nucleoprotein antibodies have been developed and validated by ELISA and WB assays.

Pan Influenza A NP Antibodies

Pan Influenza B NP Antibodies

Western Blot Analysis of Hemagglutinin Antibodies to 24 Influenza Antigens

Lane 1: 40204-V08B | H1N1 (A/Brevig Mission/1/1918)
Lane 2: 40776-V08B | H1N1 (A/Brisbane/02/2018)*
Lane 3: 40205-V08B | H1N1 (A/California/07/2009)
Lane 4: 40723-V08B | H1N1 (A/Guangdong-Maonan/SWL1536/2019)*
Lane 5: 40724-V08B | H1N1 (A/Hawaii/70/2019)*
Lane 6: 40777-V08B | H1N1 (A/Michigan/45/2015)*
Lane 7: 11675-V08B | H1N1 (A/Puerto Rico/8/34/Mount Sinai)
Lane 8: 40774-V08B | H1N1 (A/Victoria/2570/2019A/Wisconsin/588/2019)
Lane 9: 40033-V08B | H2N2 (A/Ann Arbor/6/1960)
Lane 10: 40207-V08B | H3N2 (A/Aichi/2/1968)
Lane 11: 40778-V08B | H3N2 (A/Cambodia/e0826360/2020 (H3N2)-like)
Lane 12: 40208-V08B | H3N2 (A/Hong Kong/1/1968)
Lane 13: 40753-V08B | H3N2 (A/Hong Kong/2671/2019)*
Lane 14: 40754-V08B | H3N2 (A/Hong Kong/45/2019)*
Lane 15: 40781-V08B | H3N2 (A/Hong Kong/4801/2014)*
Lane 16: 40779-V08B | H3N2 (A/Kansas/14/2017)*
Lane 17: 40499-V08B | H3N2 (A/Switzerland/9715293/2013)*
Lane 18: 40110-V08B | H7N9 (A/Anhui/1-BALF_RG6/2013)
Lane 19: 40111-V08B | H7N9 (A/Shanghai/2/2013)
Lane 20: 40783-V08B | Influenza B (B/Brisbane/60/2008)*
Lane 21: 40782-V08B | Influenza B (B/Colorado/06/2017)*
Lane 22: 40438-V08B | Influenza B (B/Florida/4/2006)
Lane 23: 40500-V08B | Influenza B (B/Phuket/3073/2013)*
Lane 24: 40755-V08B | Influenza B (B/Washington/02/2019)*

*: Antigen from 2015-2022 Influenza Vaccine Strain